MICROBIALIENI
RIASSUNTO
I microrganismi benefici sono sempre più utilizzati nell’agricoltura moderna con vantaggi significativi sulle produzioni e sulla sostenibilità del sistema agricolo. Tuttavia l’introduzione di specie non indigene come bioinoculi può comportare una loro diffusione non controllata ed effetti sulle specie microbiche endemiche di difficile analisi. In questo progetto si vuole mettere a punto un protocollo basato su sistemi di sequenziamento del DNA di terza generazione (Oxford Nanopore Technology, ONT) per il tracciamento, la quantificazione ed il monitoraggio nel suolo di bioinoculi ad azione benefica. Sarà usato un sequenziatore di DNA portatile MinION per valutare, con metodi metagenomici innovativi, la persistenza in suoli agrari di un fungo introdotto e gli effetti sulle comunità batteriche e fungine endemiche del suolo coltivato. Sempre per mezzo della tecnologia ONT, saranno valutati gli effetti del bioinoculo sulla espressione genica di una coltura modello (pomodoro da industria).
Codice Identificativo: A0375-2020-36644 del 28/10/2020
Costo totale del progetto: 149818,46 Euro
Costo totale DAFNE: 62188,00 Euro
Ruolo DAFNE: Operational Unit
Responsabile Scientifico DAFNE: Prof. Giuseppe Colla
Email: giucolla@unitus.it
Data inizio: 15/04/2021
Data fine: 16/01/2024
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