MICROBIALIENI

Monitoraggio di BIoinoculi e AnaLIsi degli effetti sulle specie ENdemIche

MICROBIALIENI

ABSTRACT

Beneficial microorganisms are increasingly used in modern agriculture with significant benefits on yields and sustainability of the agricultural system. However, the introduction of non-indigenous species as bioinocules can result in their uncontrolled spread and effects on endemic microbial species that are difficult to analyze. In this project, we aim to develop a protocol based on third-generation DNA sequencing systems (Oxford Nanopore Technology, ONT) for tracking, quantification and monitoring in soil of beneficial bioinocules. A MinION portable DNA sequencer will be used to assess, by innovative metagenomic methods, the persistence in agricultural soils of an introduced fungus and the effects on bacterial and fungal communities endemic to the cultivated soil. Also by means of ONT technology, the effects of bioinoculation on gene expression of a model crop (processing tomato) will be evaluated.

Identification Code: A0375-2020-36644 del 28/10/2020

Total project cost: 149818,46 Euro

Total cost DAFNE: 62188,00 Euro

DAFNE Role: Operational Unit
Scientific Supervisor DAFNE: Prof. Giuseppe Colla

Email: giucolla@unitus.it

Start date: 15/04/2021

End date: 16/01/2024

LINK

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PARTNERSHIP

• CNR
• Università della Tuscia, DAFNE

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