PanWheatGrain

Grain Pangenomics for Durum Wheat Sustainable Production

PanWheatGrain

ABSTRACT

Project aims are:

1. Characterizing a structured tetraploid wheat core collection, grown under optimal and low nitrogen management, for the following target traits: NUE, grain protein and starch accumulation, in relation to grain yield
2. Assembly and annotate a tetraploid wheat pangenome based on A) at golden-standard level, ten diverse, representative tetraploid wheat genomes using PacBio HiFi long reads, accurate technology B). Characterize their corresponding transcriptomes under optimal and sub-optimal N levels
3. Characterize the structure, gene content, genetic diversity and functional regulation of storage protein genes in tetraploid wheats. Define the biosynthetic/metabolic pathways and the gene regulatory networks relevant for grain protein and starch content, grain protein deviation, grain size and/or metabolic proxies/biomarkers of those traits
4. Elucidate the functional role and functional diversity at defined targeted gene families/classes including gene families controlling nutrient availability and grain filling, grain and TF that integrate growth, N assimilation and C portioning
5. Use GWAS, meta-QTL analysis and haplotype-phenotype association to identify genomic regions relevant for the genetic control of the target traits and, through an integration of functional genomics and bioinformatics prediction, obtain clues on the possible causative variants
6. Use gene editing to generate new allelic variants at key regulatory effector targets
7. Preparing adequate tools for selection/informed breeding.

Identification Code: CUP J83C20002000005

Total project cost: € 738.864,00

Total cost DAFNE: € 197.544

DAFNE Role: Operational Unit
Scientific Supervisor DAFNE: Stefania Masci

Email: masci@unitus.it

Start date: 08/05/2022

End date: 07/05/2025

LINK

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PARTNERNARIATO/PARTNERSHIP

Università di Bologna, Dipartimento di Scienze e Tecnologie Agro-Alimentari (capofila)

Università di Udine, Dipartimento di Scienze Agrarie ed Ambientali

CNR, Istituto di Biologia e Biotecnologia Agraria, Milano

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