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General Info

SUBJECTSEMESTERCFUSSDLANGUAGE
118387 - CELL BIOLOGY

CLAUDIA LORENTI GARCIA

First Semester 6BIOS-10/Aita

Learning objectives


Educational objectives
The course aims to provide a basic knowledge on the functioning of cells (of how cells function and interact, how they organize themselves in tissues), providing a reference framework for a further study of specific genetic, biochemical and molecular topics, during the Biology course.
Knowledge and understanding - Knowledge of the principles of cell biology and of the organization in tissues. Knowledge of the principles of cell structure, formation, transport and functioning of biological macromolecules in the cellular and extracellular environment. Basic knowledge of the structure of the nucleus and DNA / RNA. Basic knowledge of cell functioning from transcription to gene translation.
Applying knowledge and understanding - The basic knowledge acquired through the study of cytology will allow students to better understand the disciplinary insights of the teachings that will be carried out in the following years. Furthermore, in this course, in addition to providing a basic knowledge of cellular functioning, the knowledge relating to: ion pumps, the cytoskeleton, the extracellular matrix and cell adhesions, the transport of newly formed proteins / exocytosis and endocytosis will be particularly deepened. The students will then apply the aforementioned knowledge to the study of tissues and in particular of the neuron and nervous tissue, of muscle and muscle contraction, of the epithelium and its relationship with the connective tissue.
Making judgments - The course offers links with other disciplines of the degree program by providing an integrated, current and dynamic knowledge, susceptible of specific insights such as: a) reading of scientific material found through keyword research and also provided and shared with learners on the google-drive site see text item; b) possibility to work on the power point material provided by the teacher and available on the above link; c) possibility to participate in themed seminars organized by the teaching staff.
Communication skills - During the lessons students are invited to give their opinion and to study in groups to develop their communication skills. These skills are then verified during the ongoing exams and at the end of the training activities.
Learning skills - Students must be able to describe scientific topics related to cytology and in particular to the biology of the cell and its organization with other cells in tissues. To consider the learning of the objectives as expected, it is important to not only know the topics of the proposed program, but also to be able to connect the various topics of the program, to express them using terms, the specific terms and to apply them to themes will be taken into consideration. similar to the program. Such restitution skills will be developed through the active involvement of students in the in-depth study of the course topics

118385 - MATEMATICS AND STATISTICS

LUCA MAISTI

First Semester 9MATH-03/Aita

Learning objectives

Educational objectives
The aim of the course is to provide students with the main notions of the analysis mathematics and statistics, aimed at making students learn techniques necessary to study the functions, solve problems based on integral calculus and solve some easy differential equations. Where possible, the concepts covered will be applied to build and study mathematical models of real phenomena related to applied sciences, in particular biology.
Knowledge and understanding - Acquire knowledge the concepts of function, limits and derivability of the functions of one real variable and all the notions that allow you to study a function; the notion of integral, integration methods and the main ones applications of integral calculus; differential equations and some solving methods.
Applying Knowledge and understanding - Knowing how to use the concepts learned for solve equations and inequalities; calculate limits, derivatives, integrals and study functions; solve differential equations.
Making judgements - Be able to identify the appropriate rules to apply to solving new problems, similar to those discussed in class.
Communication skills - Students' ability to talk, reason and will be stimulated discuss the questions raised during the lessons regarding the topics treated.
Learning skills - Be able to discuss some scientific topics by building simple mathematical models.

Teacher's Profile

courseProgram

- origins and applications of mathematics
- sets and numerical sets; induction principle
- real variable functions
- properties of functions
- limits calculation and continuity
- calculation of derivatives, optimization in one variable and study of the concavity
- integral calculation
- differential equations and some models
- statistical analysis: data and representation, position and dispersion indices, correlation
- probability elements and combinatorial calculus; discrete and continuous variables

examMode

The assessment of learning will be carried out through a written test and an optional oral test. The written test involves the resolution of exercises on course topics as well as some questions, to be carried out in 2 hours. The oral test is concerned with the discussion of the written test and the course topics. The written test is considered passed with a vote of at least 18/30

books

Recommended Texts:

1. Elementi di Statistica e Matematica - Luca Secondi e Donatella Crea

2. Esercizi e Applicazioni di Statistica e Matematica - Luca Secondi, Marco di Pietro, Sara Milliani

classRoomMode

Attendance at lessons and exercises is not mandatory but strongly recommended

118389 - INORGANIC CHEMISTRY

GIORDANO PONETI

First Semester 7CHEM-03/Aita

Learning objectives

Educational objectives
The course introduces, through frontal lessons and exercises, the basic concepts of Chemistry, aiming to the understanding and to the theoretical and experimental analysis of biological phenomena. The study of stoichiometry allows to describe the quantitative aspects of chemical reactions. The knowledge of the atomic structure and the periodicity of the atomic properties is the foundation for the discussion of the different kinds of chemical bonds and interactions, of the molecular geometries as well as the nomenclature of simple chemical compounds. Chemical thermodynamics deals with the energy involved in chemical processes (both reactions and phase transitions), while chemical kinetics describes the rate of such processes, allowing to rationalise the concept of chemical equilibrium, a central topic in biochemical phenomena. Examples of fundamental chemical reactions are finally presented, like acids and bases, precipitation and redox reactions. These concepts are key to the critical understanding of the chemical aspects underlying the different topics of the degree course.
Knowledge and understanding - Knowledge of the basic principles of General Chemistry to understand the reactivity and fundamental chemical properties of matter, with particular reference to its application in Biochemistry.
Applying Knowledge and understanding - The students will be able to balance chemical reactions, estimate the heat, rate and spontaneity of a chemical process, solve problems about colligative properties, chemical, acidic and base and solubility product equilibria.
Making judgements - Gain the basic knowledge required to interpret the chemical topics discussed in the course.
Communication skills - Develop a good exposing ability (written and oral) of the acquired concepts.
Learning skills - To be able to learn and analyse the topics in different contexts and autonomously.

Teacher's Profile

courseProgram

1. Introduction
Introduction to Chemistry and to the course. Concepts of elements, compound and homogeneous and heterogenous mixtures. The atomic structure: from Democritus to Dalton. Protons, neutrons and electrons. The laws of conservation of matter, of defined proportions and multiple proportions. Atomic number and mass number, concept of isotope. Atomic masses and relative atomic masses.
2. Stoichiometry
Avogadro's number, mole and molar mass. Balance of a chemical reaction: elements of stoichiometry (yield of a reaction and limiting reagent). Classes of compounds and nomenclature. The oxidation number. Balancing redox equations.
3. The electronic structure of the atom
The Bohr model of the hydrogen atom, atomic spectra. The duality wave/particle of matter and the De Broglie equation. Heisenberg's uncertainty principle. The Schroedinger equation. Atomic orbitals, quantum numbers and electron spin. Pauli exclusion principle. Electronic configuration of the elements. Principle of Aufbau. The periodic system of the elements. Periodic properties: ionization energy, electron affinity, atomic and ionic radius. Metals, non-metals and metalloids.
4. The chemical bond
The covalent bond (pure and polar), ionic, dative and metallic bonding. Electronegativity and electric dipole moment. Valence and oxidation number. Bond properties: order, distance and energy. Classification and nomenclature of oxides and their hydrolysis products. Coordination compounds and their significance in biochemistry. Lewis structures. VSEPR model and geometry of molecules. Orbital hybridization. Sigma and pi orbitals. Resonance. Intermolecular forces: Van der Waals, London and hydrogen bond.
5. The gaseous state
Ideal gas state equation. Dalton's Law for gaseous mixtures. Density and relative density of gases and gaseous mixtures. Kinetic-molecular theory and velocity distribution. Graham's effusion law.
6. Chemical thermodynamics
Thermodynamic systems definitions: open, closed, isolated. Status functions. Reversible and irreversible transformations. Heat, work and internal energy. First principle of thermodynamics. Enthalpy and Hess law. Thermochemistry. Second principle of thermodynamics: microscopic and macroscopic definitions of entropy. Spontaneous processes. Gibbs Free energy. Temperature effects on the spontaneity of a chemical reaction. Introduction to the concept of chemical equilibrium.
7. Chemical equilibrium
Thermodynamic equilibrium in chemical reactions. Mass action law: KC and KP. Homogeneous and heterogeneous equilibria. The principle of Le Chatelier. Effect of the variation in concentration of a reagent or a product on equilibrium. Effect of variation of volume, pressure and temperature on homogeneous equilibria.
8. Chemical Kinetics
Definition of the rate of a chemical reaction. Kinetic laws and kinetic order of a reaction. Integrated kinetic laws. Order and molecularity of a reaction. Arrhenius equation and activation energy. Kinetic mechanism of reactions. Collision theory and theory of activated complex. Catalysis.
9. Physical properties of solutions
Concentration units of solutions. Solubility and thermodynamics of dissolution. Enthalpy of dissolution and effect of temperature on solubilization processes. Colligative properties: lowering of vapor pressure (Raoult's law), elevation of boiling point, depression of freezing point, osmotic pressure. Henry's law. Colloids: lyophilic and hydrophilic. Amphiphilic substances: micelles.
10. Acids and bases
General definitions (Arrhenius, Broensted-Lowry, Lewis). Strength of acids and bases and equilibrium constants. Molecular structure and relation with the acid-base properties. Water autoprotolysis, pH and pOH. Calculation of pH of acidic, basic, monoprotic and polyprotic solutions. Buffer solutions.
11. Electrochemical
Galvanic and electrolytic cells. Standard potential and electromotive force. The electrochemical series and the spontaneity of a redox reaction. Thermodynamics of galvanic cells: the Nernst equation and the equilibrium constants of a redox reaction.

examMode

The exam consists in a three-hour test, focusing on the whole program of the course, including open questions about theory, exercises, multiple-answer questions and true/false questions. Each question reports its contribution to the final mark of the test. Students attending the lessons will be given the opportunity to split the final exam in two written tests, each one on a specific part of the program.

Examples of questions to be found in the test:
1. Considering the Aufbau principle, discuss why the first period of the periodic table contains only two elements, whereas the second contains eight. Write the electron configuration of gallium (Ga).
2. Describe the effect of a catalyst on the rate of a reaction, using the Arrhenius equation.
3. Disulfur dichloride (S₂Cl₂), used in the vulcanization of rubber, is prepared by heating sulfur (S₈) in an atmosphere of chlorine (Cl₂): S₈(l) + 4 Cl₂(g) → 4 S₂Cl₂(l)
What is the theoretical yield of S₂Cl₂ in grams if 4.06 g of S₈ react with 6.24 g of Cl₂? If the actual yield of S₂Cl₂ is 6.55 g, what is the percent yield?

books

R. Chang, K. Goldsby, “Chemistry”, McGraw-Hill Education, 2020. ISBN: 8838668019

classRoomMode

Attendance to the classes is not mandatory, yet strongly encouraged.

bibliography

Recommended texts:

P. W. Atkins, L. Jones, "Chemical Principles - The quest for insight", V edition, W. H. Freeman and Co., New York
J. C. Kotz, P, M. Treichel, J. R. Townsend, "Chemistry and chemical reactivity", VII edition, Brooks/Cole, Cengage Learning
T. E. Brown , H. E. LeMay , B. E. Bursten , C. Murphy , P. Woodward, "Chemistry, the central science", XIII edition, Pearson

NOTE: The teacher will communicate at the beginning of the course the link to the additional teaching material available to the students.

118386 - ANIMAL BIOLOGY

CLAUDIO CARERE

Second Semester 9BIOS-03/Aita

Learning objectives

The course, which deals with a basic subject in the initial phase of the course of study, aims at forming the skills of critical analysis based on scientific methodology, integrating general and contextual knowledge with those concerning specific technical and methodological tools of the zoological discipline , oriented to environmental studies.
EXPECTED LEARNING RESULTS
1) Knowledge and understanding
The acquired knowledge will concern elements of general biology (cell, reproduction, genetic code, evolution); zoological techniques and methods (nomenclature, taxonomy, methods of data collection and analysis); topics of general zoology (structure and apparatus, species and reproductive isolation, ethology); biodiversity (knowledge and summary identification of the fauna with particular regard to the Italian fauna). Understanding skills will be achieved through the practice of rules and methods specific to the subject (for example, taxonomy and nomenclature rules).
2) Knowledge and comprehension skills applied
The aspects concerning the application of knowledge involve the ability to analyze and interpret scientific contributions in the field of Zoology, and to identify fauna at a summary level for environmental assessment purposes.
3) Autonomy of judgment
The exercise of critical analysis and knowledge, although at a general level, of fundamental methods and concepts will allow the ability to formulate independent assessments.
4) Communication skills
These skills will be developed through the exercise of one's own expression (interventions during the lessons) and with a brief knowledge of the methods of scientific communication.
5) Ability to learn
The notions and the use of the sources and the relative information can allow the application of the learning modalities to contexts different from those treated specifically in the course.

Teacher's Profile

courseProgram

A – General
1. Animals among living beings; 2. Definitions of Taxonomy, Identification, Classification, Faunistics, and Biogeography; 3. Taxonomic categories and taxa; 4. Analogy, homology, adaptive convergence; 5. Classification criteria and methods; 6. Phylogenetic reconstruction; 7. Species concepts; 8. Interspecific and intraspecific variability; 9. Dispersal capacity and ecological tolerance; 10. Evolutionary mechanisms; 11. Natural selection; 12. Speciation; 13. Reproductive isolation; 14. Basics of the biology of animal behaviour; 15. Rules of zoological nomenclature
B – Systems and organ systems
1. Stages of embryonic development; 2. Body cavity; 3. Symmetry; 4. Classification of the kingdom Animalia; 5. Reproduction: general information and comparison between sexual and asexual reproduction; 6. Types of Asexual Reproduction; 7. Gonochorism: Sexual Characteristics; 8. Gonochorism: Mate Recognition and Synchronization; 9. Gonochorism: Copulation; 10. Hermaphroditism; 11. Parthenogenesis; 12. Reproductive Strategies and Parental Care; 13. Digestive Systems and Feeding; 14. Feeding Strategies in Animals; 15. Functions and Structure of the Integument; 16. Colors in Animals; 17. Adaptive Coloration; 18. Functions of Respiration; 19. Physical Factors in Respiration; 20. Biological and Environmental Factors in Respiration; 21. Respiratory Structures in Animals
C – Systematics
1) PROTOZOA; 2) PORIFERA; 3) RADIATA (Cnidaria: Hydrozoa, Scyphozoa, Hexacoral Anthozoa, Octocoral Anthozoa), Ctenophora); 4) FLATHELMINTHS (Turbellarians, Trematodes, Cestodes); 5) NEMATODES; 6) ANNELLIDAE (Wandering Polychaetes, Sedentary Polychaetes, Oligochaetes, Hirudineans); 7) MOLLUSCS (Polyplacophorans, Scaphopods, Bivalves, Gastropods, Nautiloid Cephalopods, Coleoid Cephalopods); 8) CHELICERATE Arthropods (Merostomes, Arachnids: Scorpions, Spiders, Opiliones, Pseudoscorpions, Mites); 9) CRUSTACEAN Arthropods (Branchiopods, Ostracods, Copepods, Barnacles, Malacostracans); UNIRAMI Arthropods (Chilopods: Geophilomorphs, Scolopendromorphs, Lithobiomorphs, Scutigeromorphs - Insects: Thysanura, Ephemeroptera, Odonata, Orthoptera, Plecoptera, Isoptera, Blattaria, Dermaptera, Phasmids, Mantoidea, Anoplura, Heteroptera, Homoptera, Aphanictera, Diptera, Coleoptera, Lepidoptera, Hymenoptera); 10) ECHINODERMS (Crinoids, Holothurians, Ophiurids, Asteroids, Echinoidea); 11) CHORDATES (Ascidians, Agnathans, Chondrichthyes, Osteichthyes; Amphibians: Anurans, Urodelae; Reptiles: Chelonians, Saurians, Snakes; Birds; Mammals: Insectivora, Chiroptera, Lagomorphs, Rodents, Carnivores, Artiodactyls, Cetaceans).
D – Applications of zoology to environmental monitoring; behavioral analysis methodologies; assessment of the biological quality of watercourses with macroinvertebrates, with field exercises.

examMode

The exam will be conducted in accordance with the University Academic Regulations. An electronic report will be prepared and signed by the Chair of the examination board. The grade is expressed in thirtieths, with honors being possible. Passing the exam requires a grade of at least eighteen out of thirty and will result in the awarding of the corresponding university credits.
The exam will be conducted according to the following criteria: 1) assessment of knowledge of the basic elements of zoological nomenclature and zoological taxonomic methodologies; 2) question on general aspects of animal biology (Part A of the syllabus); 3) application to Part B of the syllabus; 3) practical test on animal taxa recognition (Part C of the syllabus).
In evaluating the tests and assigning the final grade, the following will be taken into account: the level of knowledge (superficial, appropriate, precise and complete, complete and in-depth), the ability to apply theoretical concepts (errors in the application of concepts, fair, good, consolidated), the ability to analyze, synthesize, and connect across disciplines (sufficient, good, excellent), the ability to use critical thinking and formulate judgments (sufficient, good, excellent), and the mastery of expression (poor, simple, clear and correct, rich and correct).

books

Any recent university textbook in Zoology, Animal Biology or Animal Biodiversity. Other material (pdf, ppt, recognition forms) will be provided by the teacher

classRoomMode

The frequency is not compulsory but it is strongly recommended

118383 - PLANT BIOLOGY

MARCELLA PASQUALETTI

Second Semester 9BIOS-01/Bita

Learning objectives

Educational objectives
The course will enable students to understand the peculiar morphological, physiological and metabolic characteristics of plants, their role in the environment and interactions with other organisms. Furthermore, it will allow to know the basic techniques for their taxonomical identification. The course will provide a framework for understanding current issues related to plants (I.e., climate changes, soil stability, desertification processes) and their potential applications. The course will provide the necessary insights to understand the ecological peculiarities of plants and their role in the Biosphere.
Knowledge and understanding - To get the knowledge regarding the basic principles of biology, physiology and ecology of plants and their potential in terms of applications.
Applying Knowledge and understanding - To understand the main ecosystem issues concerning plants with particular regard to interactions with the other biotic components including humans. To acquire the basic knowledge regarding the experimental approach to study the plant communities.
Making judgement - The ability to construe the results obtained by the various studies in botany. The ability to understand the role of the studied organisms in the environment and their influence on the biosphere.
Communication skills - Ability to describe scientific topics related to plant biology both in written and oral forms by a specific scientific/technical language.
Learning skills - Ability to use the methods acquired for studied organisms recognition and cultivation. This ability will be developed also trough the active involvement of the students in discussions during the lectures and by laboratory experiences.

Teacher's Profile

courseProgram

Introduction to the course. Peculiarities of plant organisms. Autotrophic and heterotrophic organisms. Prokaryotes and eukaryotes. Cell theory. Evolution of the eukaryotic cell (endosymbiosis).
The plant cell: plasma membrane, cytoskeleton, endoplasmic reticulum, Golgi bodies, secretory pathways. Vacuoles. Cell wall. Plastids. Cell differentiation and growth. Plant tissues: structure and function. Primary and secondary meristematic tissues. Parenchymatic tegumental, mechanical, conductor and secretory tissues.
Plant anatomy. The stem structure and function. Apical bud, cell differentiation. Primary structure: Dicotyledonous and Monocotyledonous. Differentiation of the cribro-vascular exchange (secondary meristem), secondary structure, fellogeno and felloderma. The root structure and function, primary and secondary structure, lateral and adventitious roots. Absorption of water and mineral salts. The leaf structure and function; stomatal anatomy and control of stomatal aperture.
Vegetative propagation, asexual and sexual reproduction. Life cycles: haplont, diplont, the haplo-diplont cycle of plants. Microsporogenesis and microgametogenesis, macrosporogenesis and macrogametogenesis.
The flower: the gynaeceum and the androecium; evolutionary aspects. Reproductive strategies: anemophilous and entomophilous pollination. Fertilization, development of the fruit. The seed and their dissemination.
Plant diversity, concepts of systematics, taxonomy and nomenclature. Main morphological, biological, phylogenetic and ecological aspects of Algae (Cianophyta, Glaucophyta, Euglenophyta, Cryptophyta, Haptophyta, Dinophyta, Rhodophyta, Chlorophyta, Streptophyta, Eterokontophyta), Bryophyta, Psilophyta, Lycophyta, Sphaenophyta, Pteridophyta, Coniferophyta, Ginkgophyta, Cycadophyta, Gnetophyta, Anthophyta (Dicotyledonous and Monocotyledonous). Fungi: Chytridiomycota, ex-Zygomycota, Ascomycota, Basidiomycota, Glomeromycota, mitosporic fungi.
Photosynthesis (C3, C4, CAM), photorespiration.

examMode

The student will be assessed through questions regarding the entire program carried out.

books

Pasqua, G., Abbate, G., Forni, C., & Acosta, A. T. (2011). Botanica generale e diversità vegetale. Piccin.

Ray F Evert Susan E Eichhorn. Biologia delle piante di Raven. (2013). Settima edizione italiana condotta sull'ottava edizione americana.Trad. di A. Virzo, V. Vona, R. Castaldo, A. Basile, C. Rigano, D. Rigano, R. Nazzaro, P. Caputo, A. Pollio, G. Siniscalco Gigliano, V. Spagnuolo. Zanichelli.

mode

Lectures (56 h), laboratory experiences and educational excursions (16h).

classRoomMode

Lesson attendance is highly suggested.

bibliography


Burki, F. (2014). The eukaryotic tree of life from a global phylogenomic perspective. Cold Spring Harbor perspectives in biology, 6(5), a016147.

Katz, L. A., Grant, J. R., Parfrey, L. W., & Burleigh, J. G. (2012). Turning the crown upside down: gene tree parsimony roots the eukaryotic tree of life. Systematic biology, 61(4), 653-660.

Pasqualetti, M., Giovannini, V., Barghini, P., Gorrasi, S., & Fenice, M. (2020). Diversity and ecology of culturable marine fungi associated with Posidonia oceanica leaves and their epiphytic algae Dictyota dichotoma and Sphaerococcus coronopifolius. Fungal Ecology, 44, 100906.

Pasqualetti, M., Barghini, P., Giovannini, V., & Fenice, M. (2019). High production of chitinolytic activity in halophilic conditions by a new marine strain of Clonostachys rosea. Molecules, 24(10), 1880.

118424 - ENGLISH

Second Semester 4ita

Learning objectives

Educational objectives - The student can: understand texts that consist mainly of high-frequency everyday or job-related language; understand the description of events, feelings, and wishes in personal letters; understand the main points of clear standard speech on familiar matters; enter into a conversation on topics that are familiar, of personal interest or pertinent to everyday life; connect phrases in a simple way in order to describe experiences and events, his/her dreams, hopes and ambitions; briefly give reasons and explanations for opinions related to scientific topics as well; write simple connected text on topics that are familiar or of personal interest; write personal letters describing experiences and impressions, and short stories sticking to specific hints.
Knowledge and understanding -The learner is able to know and understand the topics related to the syntax and vocabulary of the English language for a B1 level, which concern the structures to be used in everyday communication. He/she is also able to understand the key points of topics relating to their specific field of study.
Applying Knowledge and understanding -The learner is able to interact with ease, without errors and misunderstandings, in everyday situations where the language of communication is English. He/she is able to use the tools and vocabulary learned related to their field of study.
Making judgements - The learner is able to independently deepen, through information and communication technologies, what he/she has learnt with regard to everyday aspects of language use but especially with regard to knowledge acquired in his/her specific field of study
Communication skills - The learner has acquired the ability to produce written texts, in a clear and complete manner, on topics of everyday life but also on subjects related to their specific field of study.
Learning skills - The learner is able to act independently to deepen and integrate the knowledge acquired during the course, expanding the specialised lexicon, writing and communication strategies to be implemented in an English language conversation.

118390 - PHYSICS AND LABORATORY

CHIARA BALDACCHINI

Second Semester 9PHYS-06/Aita

Learning objectives

Educational objectives
The aim of the course is to provide students with the fundamental concepts of physics, at the same time providing the logical-deductive tools necessary to achieve a full understanding of the issues presented. Students will acquire the basic principles of the scientific method that combines the experimental approach with a mathematical-deductive approach. Particular attention will be paid to the critical and historical analysis of the concepts on which a scientific theory is based.
Knowledge and understanding - To have developed the knowledge of the fundamental principles of Physics and of the relative methodologies.
Applying Knowledge and understanding - Knowing how to use the concepts learned even in contexts different from those presented.
Making judgements -Develop critical analysis skills and be able to solve new problems even if similar to those discussed in class.
Communication skills - Students' ability to discuss the implications of concepts presented in class and the possible questions that may emerge from the topics discussed will be stimulated.
Learning skills - Being able to discuss fundamental scientific topics of Physics and its applications. This skill will be developed and verified by involving students in oral discussions in the classroom.

Teacher's Profile

courseProgram

Physics (56 hours)
The experimental method, dimensional analysis, unit of measurement, the International System.
The experimental measure, measuring instruments, random and systematic errors.
Description of motion: vectors, kinematics in one dimension, kinematics in two dimensions, projectile motion.
Force, mass and Newton's laws. Weight force, normal force, friction.
Elastic force, elasticity and fracture.
Circular motion and Gravitation Law.
Work and Kinetic Energy Theorem. Potential energy, conservative and dissipative forces, conservation of mechanical energy. Power and efficiency.
Momentum and its conservation, impacts and center of mass.
Rotational motion, torque, moment of inertia, angular momentum and its conservation.
Statics: force couples, levers and bodies in equilibrium.
Oscillations and vibrations, simple harmonic motion, the simple pendulum. Waves and their propagation. Sound.
Fluid Statics: density, pressure, compressible and incompressible fluids, Stevin's law, Pascal's principle, Archimedes' principle.
Fluid Dynamics: continuity equation, Bernoulli's equation, Torricelli's theorem, lift, viscosity, Poiseuille's equation. Surface tension, contact angle, and capillarity.
Temperature, ideal gas law, kinetic theory of gases, diffusion.
Heat and calorimetry. Heat transfer and Stefan-Boltzmann law.
Laws of thermodynamics, heat engines, entropy.
Electric charge, Coulomb's law, and electric field. Electric potential and electric potential energy. Electric dipole and its moment.
Capacitors, capacitance, and dielectrics.
Voltage sources, electric currents, Ohm's law and resistance, power and Joule effect. Alternating current. Direct current circuits.
Electrical transport in the human body: the RC model of the cell membrane, membrane potential, action potential.
Magnetism, magnetic field, magnetic force, mass spectrometry. Ampére’s Law, Biot-Savart Law, magnetic dipole moment of a current loop.
Electromagnetic induction and Faraday-Lenz’s law.
Electromagnetic waves and their spectrum.
Light: geometric optics (reflection, refraction and dispersion) and physical optics (polarization, interference, diffraction). Spectroscopic techniques. Optical instruments (human eye and microscope). Resolution and Rayleight’s Criterion.
Quantum theory and Photoelectric effect.
Atomic models. De Broglie's length and hypothesis.
Quantum mechanics and quantum numbers.
Electronic and scanning probe (AFM, STM) microscopies.
Nuclear physics and radioactivity. Law of radioactive decay. Natural radioactivity, nuclear reactions, fission and fusion. Ionizing radiation and biological effects of radiation. Measurement of ionizing radiation doses.

Physics laboratory (16 hours)
Evaluation of uncertainties in direct measurements. Significant figures. Discrepancy.
Propagation of errors: sum and difference, product and quotient.
Calculation of errors for functions of a variable and for functions of several variables.
Errors in repeated measurements: average as best estimate, standard deviation, standard deviation of the mean. Gaussian distribution.
Least squares method.
Correlation coefficient.
Binomial distribution.
Poisson’s distribution.
Chi-square test.
Graphs: histograms, scatter plots, linear scale, semilogarithmic scale, logarithmic scale.
Laboratory experiences:
- Theory of Errors
- Harmonic motion
- Geometric optics
- Radioactivity

examMode

The exams consist of a written test that covers the entire program carried out, including laboratory activities. In the written test some problems are assigned. Each problem can be solved according to the methodologies, the understanding of the physical principles and the examples presented in class. In addition to questions that require application and development of formulas, as well as numerical resolution, open questions can be introduced, to which the student must answer concisely. The mark of the written test is expressed in 27ths.
The four laboratory reports will be evaluated, and each will be assigned a maximum grade of 1.
The final grade of the exam will be obtained by adding the grade of the written exam with that of the four reports.
The exam is passed if the student receives a total score of at least 18/30. The student can take the oral exam if he intends to improve his grade. If the oral test is also taken, the appeal mark is the average of the mark obtained from the written test and the reports and the oral test.
The calendar of appeals is published on the University website, and registration for exam appeals is made through the same website.

books

- Giancoli "Physics" (edition with Modern Physics - 3rd Edition) - Casa Editrice Ambrosiana
- Taylor "Introduction to Error Analysis: The Study of Uncertainties in Physical Measurements" - Zanichelli

Supplementary materials will be provided via the course page on the Moodle portal.

classRoomMode

Attendance at lectures is optional.
Attendance at the laboratories is mandatory and it will be evaluated in the final mark. For each experience the students will have to produce a report, individual or group depending on the number of students participating in the activity.

bibliography

- Giancoli "Physics" (edition with Modern Physics - 3rd Edition) - Casa Editrice Ambrosiana
- Taylor "Introduction to Error Analysis: The Study of Uncertainties in Physical Measurements" - Zanichelli

SUBJECTSEMESTERCFUSSDLANGUAGE
118391 - ORGANIC CHEMISTRY

BRUNO MATTIA BIZZARRI

First Semester 7CHIM/06ita

Learning objectives

The course introduces the concepts and the experimental approaches of organic chemistry, working on the consolidation of principles acquired in the field of physics and general and inorganic chemistry to advance the knowledge of carbon chemistry. In the first part of the course, the cultural and practical bases for understanding the structure of organic molecules will be provided, paying particular attention to the existing relationships between the chemical structure and the chemical-physical and biological properties associated with them. The different physical hybridization states of the carbon will allow the three-dimensional vision of the molecules, facilitating the understanding of their role in the cell. The second part of the course is dedicated to the application of properties in the context of chemical reactivity. The student will have the opportunity to have answers to some of the key questions in his study: why do molecules react? What are the experimental factors that control the kinetics of the reactions? When is a reaction under thermodynamic control rather than kinetic? How is it possible to synthesize complex molecules from simple reagents? What is the impact of organic chemistry on the environment and how can it be reduced? This knowledge will allow the student to undertake subsequent study courses with strong structural and molecular expertise.

B) EXPECTED LEARNING RESULTS
Knowledge of the principles governing the formation of the chemical bond, using traditional theories (valence bond theory) and advanced theories (theory of molecular orbital and quantum mechanics ). Knowledge of nomenclature and classification (theory of functional groups) of organic molecules, with particular attention to the association between the family of organic molecules and biological and chemical-physical properties. Knowledge of the reactivity of organic molecules and experimental parameters capable of controlling thermodynamics and kinetics of organic transformations. Knowledge of the relationship between organic molecules and the origin of life.

In addition to the knowledge gained through the study of organic chemistry, students will be able to apply the acquired concepts for the resolution of practical exercises related to the identification and classification of substances based on Their activity on the body, the effect of chirality on pharmacological activity, the possibility of separating organic isomers and the general methodologies for their analysis and their recognition.

Making judgments: The course offers links to other disciplines (Physics, General Chemistry, Biochemistry, Molecular Biology, Computational Chemistry and Genetics) by providing an integrated knowledge. The student's critical judgment will be stimulated by constantly referring to the reading of recent studies published in scientific journals, questioning the current issues related to some of the core concepts of the discipline. Thanks to the multi-disciplinary and interdisciplinary nature of organic chemistry, it will be also possible to link the acquired concepts to other disciplines, allowing the student to form his own autonomy of judgment about the effectiveness of an integrated scientific approach.

Communication skills: At the end of each part of the course, the students will be invited to form working groups to develop solutions and compete with others in solving practical exercises. The educational gain is aimed at increasing the communication skills and the ability to know how to work in a group, all aimed at consolidating the acquired concepts.

Learning Skills: Students' learning abilities will be evaluated during the course of the course by exonerary tests that will allow you to individually monitor the maturation state of the knowledge, highlighting the student's ability to return.

Teacher's Profile

courseProgram

Module A STRUCTURE
Alkanes and cycloalkanes. Introduction. Structure. Sp3 hybridization. Nomenclature. Physical Properties. Isomerism of structure. Conformational analysis (ethane, cyclohexane). Stability of cycloalkanes (angle strain, torsional strain, tension steric). Derivatives of cyclohexane (stereoisomery cis-trans). Bicyclic alkanes and polycyclic. Natural role and applications of alkanes.

Alkenes. Introduction .. Structure. Sp2 hybridization. Nomenclature. Physical Properties. Stability (heat of hydrogenation, heat of combustion). Nomenclature system (E) - (Z) for alkenes. Cicloalcheni.Funzione biological.

Alkynes. Introduction. Structure. Sp hybridization. Nomenclature. Physical Properties.

Functional groups and classes of organic compounds. Alkyl halides. Alcohols. Ethers. Amines. Aldehydes and ketones. Carboxylic acids. Esters and amides. Introduction. Structure. Nomenclature. Physical Properties.

Stereochemistry. Introduction. Chirality of carbon. Enantiomers and chiral molecules. Graphical representation. Nomenclature (R) (S). Optical activity (specific optical rotation, definition of racemate, optical purity). Diastereoisomers (meso compounds). Chiral molecules without chiral carbons.

Aromatic compounds. Introduction. Benzene. Structure and stability. Huckel rule. Other aromatic compounds. Nomenclature of benzene derivatives. Induction phenomena and resonance. Heterocyclic aromatic compounds. The aromatic compounds in biochemistry.

Carbohydrates. Classification. Monosaccharides. Mutarotation and formation of glucosides. Configuration D or L. Disaccharides. Polysaccharides.

Protein. Structure of the amino acids. Nomenclature. Peptide bond. Oligopeptides.

Nucleic acids. Purine and pyrimidine nucleic bases. Nucleosides. Nucleotides. Oligonucleotides.

Lipids.

Form B. THE REACTIVITY '

Reactions of alkanes and cycloalkanes. Chlorination of methane. Free radicals. Stability and structure. Thermodynamics and kinetics. Halogenation of higher alkanes.

Ionic reactions of substitution and elimination. Formation of carbocations. Stability and structure. Nucleophilic substitution reactions. SN2 reaction. SN1 reaction. Mechanisms and trends stereochimici.Effetti solvent and leaving group. Elimination reactions. E1 reaction. E2 reaction. Competition between substitution and elimination. Substitution reactions and elimination of biological interest.

Reactions of the C = C double bond. Addition reaction. Addition of hydrogen halides. Markovnikov rule.

examMode

The exam consists of a written test followed by an oral test. The written test consists of 5 graphic and numerical exercises relating to the topics covered in the course. Students who have obtained the minimum of 18/30 enter the oral test. The oral test consists in the discussion of the results of the written test with possible insights on other topics of the program related to them.

books

-Chimica Organica Essenziale 2a Edizione a cura di Bruno Botta (ediermes)
-Guida Ragionata allo Svolgimento di Esercizi si Chimica Organica. D'Auria, Scafati, Zampella (Loghia)

mode

Frontal lessons in the classroom at the Civitavecchia campus

classRoomMode

Attendance is not mandatory but strongly recommended

bibliography

-Chimica Organica Essenziale 2a Edizione a cura di Bruno Botta (ediermes)
-Guida Ragionata allo Svolgimento di Esercizi si Chimica Organica. D'Auria, Scafati, Zampella (Loghia)

OPTIONAL GROUP - -- -
MOLECULAR METHODOLOGIES APPLIED TO ENVIRONMENT

GIUSEPPINA FANELLI

Second Semester6BIO/11ita

Learning objectives

Educational objectives
The course of molecular methodologies applied to environmental research aims to provide students with an overview of the biomolecular and analytical instrumental techniques used for environmental studies. Knowledge will be given to develop the knowledge of environmental influences on the biology of living organisms and on humans. Students will acquire conceptual tools to understand the methods for the study of the effects of pollutants on human health and the environment and for the evaluation of toxicological risk.
Knowledge and understanding - At the end of the course the students will have to know the modern techniques of molecular biology used in the environmental field. They will understand the principles underlying the main molecular and analytical methods for the study of environmental pollutants. They will be able to correctly use the appropriate terminology used in molecular and analytical instrumental methodologies. They will be able to describe the structural elements of the main tools of a biochemical / molecular laboratory (liquid chromatography, spectrophotometer, mass spectrometer). They will know the classification of environmental toxic chemicals and their effect on humans.
Applying knowledge and understanding - Mastery of analytical and molecular methods for the study of genetic-environmental variability. Ability to identify the analytical-molecular techniques to be applied in different cases. Ability to apply techniques related to the identification of environmental pollutants.
Making judgments - To have a concrete ability to integrate basic biomolecular knowledge and manage the complexity of problems in the environmental application field.
Communication skills - Ability to expose the acquired knowledge. The student must be able to present logically, concisely and rigorously, in various forms and with different tools, objectives, concepts, data and procedures of work or experimental analysis.
Learning skills - Develop autonomous learning skills in the field of the discipline in question to increase their knowledge, constantly updating and keeping informed about new developments and bio-molecular methods used in the environmental field.

Teacher's Profile

courseProgram

Exposure to environmental contaminants (Environmental pollution, Air pollution, Water pollution, Soil pollution ). Pollutants in the environment: origin, diffusion, accumulation. Classification of toxic chemicals of environmental relevance. Toxic heavy metals Toxic organic compounds (dioxins, furans and PCBs Polycyclic aromatic hydrocarbons). Toxicity of environmental contaminants towards cellular components, hints of genotoxicity. Genes and tumors, heredity of cancer risk. Molecular aspects of tumorigenesis. Cell death, apoptosis and necrosis in response to genotoxic agents. Molecular methods for studying the environment. PCR, agarose gel electrophoresis, cloning, sequencing. PCR in real time. Application of molecular techniques for the study and isolation of environmental DNA, DNA/ RNA Stable isotopic probe. Fluorescent hybridization in situ (FISH, CARD-FISH, Raman-FISH, Nano-SIMS). Microarray.
Analytical methodologies for the determination of all classes of environmental pollutants: liquid chromatography, gas chromatography, mass spectrometry for the quantitative and qualitative analysis of environmental pollutants.
The techniques -omics : metabolomics. of proteomic mass spectrometry coupled to database analysis.
Journal club with literature reading in the environmental field and use of molecular methodologies

examMode

The purpose of the oral test is to verify the theoretical basis of molecular biological methodologies relevant to the study of the environment.
The evaluation of the examination will particularly take into account the articulation, precision and significance of the acquired theoretical content treated in an appropriate language.

books

- Francesco Amaldi Piero Benedetti Graziano Pesole Paolo Plevani
Tecniche e metodi per la biologia molecolare
- Mauro Maccarrone: Metodologie biochimiche e biomolecolari
Strumenti e tecniche per il laboratorio del nuovo millennio

Teacher's notes and teaching materials are provided during classes.
At the beginning of the course, the teacher will provide a link to download the teaching material.

mode

Teaching is proposed through frontal lessons (48 hours). Learning materials will be made available to students prior to each lesson. The space will be dedicated to the analysis of scientific papers or normative texts which apply molecular methodologies in the field of the environment.

classRoomMode

The teacher recommends student involvement with shared discussion and analysis of the subjects presented. Students are encouraged to engage with the teacher throughout the lesson. Non-regular students are required to study the texts indicated in the bibliography and may consult the material produced by the teacher, such as the students present. All students requesting clarification should contact the teacher to make an appointment.

bibliography

The teaching material will be available on the Moodle platform. Non-attending students are encouraged to contact the professor for information on the program and on any additional bibliography.

HYDROBIOLOGY

FRANCESCO CERINI

Second Semester6BIO/07ita

Learning objectives

Educational objectives
The aim of the course is to provide students with the useful information to learn the theoretical bases of classical and modern hydrobiology and the theoretical and experimental approaches that have enabled their development today. Expanding knowledge on aquatic organisms from a trophic-functional point of view. Students will have to learn the logic of ecosystem analysis of aquatic environments and the methodologies suitable for evaluating structures and functions of ecosystems in aquatic environments. Notions will be provided to understand the most recent developments in the ecosystem of aquatic environments (food web theory, niche theory, network analysis), and an understanding of the importance of managing these environments will be stimulated. The course aims to provide the basis for further in-depth studies in the fields of inland water ecology and community ecology.
Knowledge and understanding - Having developed knowledge of the principles and ecological laws that underlie the ecosystem functioning of aquatic environments. Having acquired the notions of hydrobiology useful for undertaking ecosystem analyzes of the various environmental types of inland waters. Ability to
Applying Knowledge and understanding - Knowing how to use the notions learned in class and developed in the exercises to interpret the structures and functions of the various aquatic organisms and to solve problems in the various fields of hydrobiology. Judgment autonomy. Being able to identify the theoretical and experimental paths to be applied to the resolution of new problems even if similar to those discussed in class.
Communication skills - Students' ability to talk, reason and discuss the questions raised during the lessons on the topics will be stimulated.
Learning skills - Being able to discuss scientific issues related to hydrobiology and in general the academic ecology of aquatic environments also in its management applications and in its theoretical and experimental implications. This skill will be developed and tested by involving students in classroom discussions.

Teacher's Profile

courseProgram

Introduction to the aquatic environment: Chemical and physical properties of water, Water cycle, movement of heat, light and nutrients in water.

Freshwater environments and their characterisation: River environments. Lacustrine environments. Transitional aquatic ecosystems. Wetlands. Physiography and conservation issues. Other water environments (rivers and underground lakes, high altitude springs, hydrothermal springs, interstitial waters). Semi-natural and artificial environments.

Fauna and flora of inland waters: Overview of evolution and adaptations to the water medium and the different chemical-physical characteristics of water. Trophic-functional classification of aquatic organisms. Microorganisms, algae, fungi and plants. Protozoa. Invertebrates (Cnidarians, Bryozoans, Platelminths, Nematodes, Annelids, Crustaceans, Insects). Vertebrates (Amphibians, Reptiles, Mammals, Birds, Bony and Cartilaginous Fish)

Ecology of inland waters: Interactions between species. Community and metacommunity ecology and aquatic trophic networks. Theory and case studies. Elements of sampling techniques.

Conservation, management and monitoring of freshwater environments: Threats to inland water systems. Bioindication and ecological indices. Monitoring and management.

examMode

The examination is conducted in the form laid down in Article 23 of the University Teaching Regulations. Minutes are taken of its proceedings, signed by the chairman and members of the board and by the student examined. The grade is expressed in thirtieths, with honours where appropriate. Passing the exam requires a mark of no less than eighteen/thirty-eighths and entails the award of the corresponding university credits. In assessing the test and in awarding the final grade, account will be taken of: the level of content knowledge demonstrated (superficial, appropriate, precise and complete, complete and thorough), the ability to apply theoretical concepts (errors in applying concepts, discrete, good, well established), the ability to analyse, summarise and make interdisciplinary connections (sufficient, good, excellent), the ability to make a critical sense and formulate judgements (sufficient, good, excellent), the mastery of expression (poor, simple, clear and correct, safe and correct exposition). The question will concern concepts and base knowldge addressed during the lessons, with the free choice to present a study case (e.g. a scientific paper) regarding freshwater ecology themes, from which the examination discussion can start.

books

Walter, K., Dodds, W. H. I. L. E. S., & Matt, R. (2017). Freshwater ecology: concepts and environmental applications of limnology. ELSEVIER ACADEMIC Press.

ORNERIS G., PASCALE M., PEROSINO G.C., ZACCARA P. (2013). Lezioni di Idrobiologia Editore: Regione Piemonte, Direzione Agricoltura - settore caccia e pesca (https://greencrestdotblog.files.wordpress.com/2018/01/2008-lezioni-di-idrobiologia-le-acque-continentali.pdf)

classRoomMode

Attendance is optional, but students are strongly encouraged to attend the lectures; I will explain methods of researching scientific bibliography, which are very useful for the final examination project (optional), and useful in general for a scientific career. In addition, class discussions on course topics useful for further study will be stimulated.

INTRODUCTION TO EARTH SCIENCE

CHIARA SBARBATI

Second Semester6GEO/05ita

Learning objectives

Educational objectives
The objectives of the course are transmission of the basics of geology useful to properly frame the environmental themes. The course introduces the composition of the Earth and focuses attention on the lithogenetic cycle. The exogenous and endogenous processes that give rise to formation of rocks and their structure constitute central themes of the course.
Knowledge and understanding - At the end of the training activity, the student will acquire knowledge about the inner constitution of the Earth and the main endogenous and exogenous processes that characterize the Planet. The student will be able to distinguish the most common types of rocks by linking them to their origins. The student will be able to understand the principles of stratigraphy and the main geological structures.
Applying knowledge and understanding - At the end of the training activity, the student will have to demonstrate that he is able: to distinguish and classify the main types of rocks, to read and interpret geological map.
Making judgments -Through the exercises on geological maps, the student will acquire autonomy of judgment based on the acquired knowledge, analyzing different Italian areas.
Communication skills -The student will acquire an appropriate technical language during the lessons and exercises on rocks classification and geological maps. Communication skills will be verified during examination.
Learning skills - The student must demonstrate his ability to investigate geological areas which differ from those considered during the course. The student must be able to analyze the essential geological features of an area.

Teacher's Profile

courseProgram

Shape and dimensions of the Earth. The internal structure of the Earth: core, mantle and crust. Lithosphere and asthenosphere. Notes on plate tectonics.
Elements of mineralogy: main physical and chemical properties, main minerals of rocks.
Magmatic processes and igneous rocks. Magmatic consolidation. Structure and texture of igneous rocks. Methods of classification of intrusive, extrusive and hypabyssal rocks. Characteristics of the main igneous rocks. Volcanism: types of eruptions and types of volcanoes. Pyroclastic rocks.
Exogenous processes and sedimentary rocks. Sedimentary processes: weathering, transport, sedimentation and diagenesis. Classification and characteristics of sedimentary rocks: silicoclastic; carbonatic; evaporitic; other chemical and biochemical rocks, residual rocks. Properties of sedimentary rocks. Principles of stratigraphy.
Metamorphic processes and metamorphic rocks. Metamorphic factors: temperature, pressure and fluid phase. Texture and structure of metamorphic rocks. Metamorphic facies and types of metamorphism. Main metamorphic rocks.
Outline of structural geology: folds, faults and thrusts.
Earthquakes: causes, mechanisms and distribution of earthquakes.
Overview of the chronology of Earth.
Topographical maps. Geological maps.
Erosive processes, rivers and river erosion, wind erosion, glacial erosion, coastal erosion, landslides.

examMode

The assessment consists of an oral test and takes place in about 30 minutes. The exam topics are those covered during the course. In particular, classification of rocks, principles of stratigraphy and structural geology, exogenous and endogenous processes, topographic and geological maps will be the exam topics. The test will also assess the candidate’s ability to apply knowledge to environmental contexts, technical language and scientific curiosity. The exam evaluation will be based on the oral exams results, with a score awarded out of thirty according to the "Regolamento didattico di Ateneo".

books

Grotzinger J.P., Jordan T.H. (2016) Capire la Terra. Zanichelli, Bologna.

classRoomMode

The attendance is not mandatory but is highly recommended.

118406 - CHEMICAL MONITORING LABORATORY

ELISA DE MARCHI

First Semester 6CHIM/06ita

Learning objectives

Educational objectives
The course introduces and deepens the concepts and main experimental approaches on the chemical aspects of environmental monitoring.
Knowledge and understanding - Knowledge of the main instrumental methods for qualitative and quantitative chemical analysis of organic and inorganic substances present in the environment. The knowledge will focus in particular on the chemical monitoring of water and soil;
Applied knowledge and understanding - In addition to theoretical knowledge acquired through the study of chemical analysis methods, students will be able to apply the main concepts studied to the analysis of samples during practical exercises taken from real situations;
Making judgements - The course offers connections with other disciplines in the Degree Course in Environmental Biological Sciences (including physics, general chemistry, and organic chemistry) providing integrated knowledge for understanding the methodological aspects of environmental chemical monitoring. Knowledge and practical application will allow autonomy of judgment and planning skills;
Communication skills - Knowledge and practical application will also be useful in developing communication skills through the reading and interpretation of instrumental data obtained;
Learning skills - Learning ability will be evaluated through exercises concerning theory and interpretation of instrumental data.

Teacher's Profile

courseProgram

The course aims to introduce and explore the main instrumental chemical analysis techniques applied to environmental monitoring of natural matrices, integrating theoretical classroom background with guided exercises and practical laboratory activities.

Theoretical Section (4 ECTS / 32 hours):
- General Framework and Sampling: Logical chain of environmental chemical monitoring, regulatory framework (e.g., DM 260/2010, Directive 2000/60/EC), and quality standards. Sampling strategy planning (representativeness, sampling, and sample preservation) and environmental matrices.
- Sample Preparation: Extraction and purification techniques. Matrix pretreatment for metal analysis: mineralization and acid digestion techniques (open-vessel and microwave-assisted).
- Analytical Data Quality and Method Validation: Limits of detection (LoD) and quantification (LoQ), sensitivity, robustness, recovery, and use of certified reference materials (CRMs) (DM 260/2010, Annex 1, pp. 29-30). Analytical uncertainty (systematic and random contributions), compliance with legal limits, and expression of analytical results.
- Chromatography and Mass Spectrometry: Chromatographic theory (partition coefficient, resolution, theoretical plates). Chromatographic modes (adsorption, partition, ion exchange, size exclusion). Instrumental methods: Gas Chromatography (GC) and High-Performance Liquid Chromatography (HPLC). Mass Spectrometry (MS).
- Atomic and Molecular Spectroscopy: Electromagnetic spectrum, radiation-matter interaction, Lambert-Beer law, and chemical structure effect on absorption. UV-Visible and Infrared (FT-IR) spectrophotometry. Atomic Spectroscopy: atomic absorption (F-AAS, GF-AAS), Atomic Emission Spectroscopy (ICP-OES / AES), and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). NMR Spectroscopy (chemical shift, multiplicity, spin-spin coupling, and coupling constants).

Laboratory Exercises (2 ECTS / 16 hours):
- Guided review of domain-specific scientific literature.
- Execution of extraction and purification techniques.
- Sample preparation for HPLC and/or GC-MS analysis.
- Calibration curve construction and data processing of chromatograms, MS spectra, and NMR spectra.

examMode

The exam consists of a written test aimed at verifying the acquisition of theoretical concepts and the ability to apply them.

books

Recommended textbooks:

1. Colin Baird, Michael Cann - "Environmental Chemistry" - Zanichelli, 2013 [ISBN: 9788808173782]

2. Robert M. Silverstein, Francis X. Webster, David J. Kiemle - "Spectrometric Identification of Organic Compounds" - Casa Editrice Ambrosiana (Exclusive distribution by Zanichelli), 2016 [ISBN: 9788808183507]

3. Mario D'Ischia - "La chimica organica in laboratorio" (Organic Chemistry in the Laboratory) - PICCIN Nuova Libraria, 2003 [ISBN: 8829916218]

classRoomMode

Attendance is not mandatory, but strongly recommended.

- - ELECTIVE MODULE

First Semester 12ita
118395 - GENETICS AND ENVIRONMENTAL MUTAGENESIS

ELENA PACCOSI

Second Semester 9BIO/18ita

Learning objectives

Educational objectives
The aim of the course is to provide students with the information necessary for understanding the theoretical foundations of classical and modern genetics and the experimental approaches that have allowed their definition. Students must learn the logic of formal genetic analysis and the methodologies of genetic dissection of biological phenomena. They will have to know how to connect the concepts of genotype and phenotype and the interaction of these with the environment. A relevant chapter of the course will be dedicated to the students' acquisition of the concepts of mutation, mutagenesis and repair of genetic damage; as well as the impact that these processes have in the generation of syndromes, in the onset of degenerative diseases and in the evolution of populations. The notions will be provided to understand the paradigm shift that took place in the post-genomic era, and the understanding of the importance of both eukaryotic and prokaryotic model systems will be stimulated.
Knowledge and understanding - To have developed the knowledge of the principles of formal genetics: Mendelism, sex-linked inheritance, mapping of genes in eukaryotes and prokaryotes, mutations, regulation of gene expression in prokaryotes and eukaryotes, genetics of populations. Having acquired the basic notions of Environmental Mutagenesis and Molecular Genetics and having understood the potential of post-genomic analysis.
Applying Knowledge and understanding - Knowing how to use the notions learned in class and developed in the exercises to interpret the patterns of inheritance and to solve problems in the various fields of Genetics.
Making judgements - Being able to identify the appropriate rules of Genetics to apply to the resolution of new problems even if similar to those discussed in class.
Communication skills - The students' ability to speak, reason and discuss the questions raised during the lessons regarding the topics covered will be stimulated.
Learning skills - To be able to discuss scientific issues related to Genetics also in its medical applications and in its evolutionary implications. This skill will be developed and tested by involving students in oral discussions in the classroom.

Teacher's Profile

courseProgram

Program:
Mendel's genetic analysis:
genetic variability;
the law of segregation;
the law of independent assortment;
genes and alleles: the concept of polymorphism.

Extensions of Mendelian analysis:
dominance relationships;
multiple allelia;
pleiotropy;
multifactorial inheritance,
inheritance of quantitative characters.
Chromosomal theory of inheritance:
mitosis and meiosis;
sex-linked inheritance;
meiosis and mendelism.

Association and recombination:
segregation of genes located on the same chromosome;
crossing over-over;
chi-square test
genetic maps;
three-point assay.

The physical basis of inheritance, DNA:
identification of genetic material;
structure; *
replication; *
recombination.

The function of the gene:
genetic dissection through mutations
the one gene one enzyme hypothesis;
complementation;
fine structure of the gene;
the genetic code;
transcription; *
translation. *

The eukaryotic chromosome
structure and organization of chromatin;
the radial loop model;
structure and function of the telomere and centromere;
the organizing regions of the nucleolus (NOR).

Mutations:
mutagenesis and mutagens;
repair of genetic damage.
Chromosomal mutations (of structure; mobile elements; of number).

Genetic Analysis of Bacteria and Viruses *:
the bacterial chromosome;
isolation of mutants;
gene transfer in bacteria:
natural and artificial transformation;
conjugation;
genetic maps in bacteria.
transduction

Gene regulation in prokaryotes:
negative and positive regulation;
the lac operon;
the tryptophan operon;
attenuation.

Gene regulation in eukaryotes:
regulation of transcription;
post-transcriptional regulation;
the role of chromatin structure;
epigenetic inheritance;
euchromatin and heterochromatin;
variegated position effect (VPE);
X chromosome inactivation in mammals.

Population genetics:
gene and genotype frequencies
Hardy-Weinberg equilibrium;
evolution of populations and species.

* These topics are treated only from the point of view of Genetics, leaving aside the
biochemical, molecular and microbiological aspects that are treated in other courses.

examMode

A final written test consisting of 4 exercises of increasing difficulty (1.5 points are assigned to the two easiest and 3 to the most complex ones) and 3 theoretical questions (7 points
each) that focus on the entire course. The written test is considered passed if the student obtains a grade equal to or higher than 18/30. In this case, the grade can be confirmed by the student, who may eventually take an optional oral test to improve it.

books

Main reference texts:
Anthony Jf Griffiths et al., Genetics. Principles of formal analysis. Zanichelli
Lucia Migliore, Genomics and environmental mutagenesis. Zanichelli

classRoomMode

in presence

118396 - BIOLOGICAL CHEMISTRY

NICOLO' MERENDINO

Second Semester 7BIO/10ita

Learning objectives

Educational objectives
The main objective of the course is to provide students with the concepts necessary to understand the biological phenomena and the energy variations connected to them and the metabolism. the course also aims to provide knowledge regarding the structure and function of the main biological molecules: proteins, carbohydrates and lipids as well as knowledge on cellular metabolism and its regulation. Finally, the course will introduce students to knowledge to describe the complex phenomena of communication, interaction and control of cellular and tissue functions with biochemical and molecular language.
Knowledge and understanding - Having developed knowledge of the principles of biochemistry in relation to carbohydrates, lipids and proteins; know the complex phenomena of communication, interaction and control of cellular and tissue functions and aims to highlight the relationships between structure and function of the main classes of biological molecules, the metabolic regulation at the molecular and cellular level; to make known the mechanisms that underlie the enzymatic functions.
Applying Knowledge and understanding - Knowing how to use the information learned in class to be able to deal with biochemistry issues in a strictly scientific way; Furthermore, students are expected to be able to apply the knowledge acquired from the course in research, in the biotechnological industry and in the sector of biological and biochemical analyses.
Making judgements -Being able to identify and understand the scientific mechanisms that underlie biochemistry in order to be able to interpret and make adequate judgments regarding the problems relating to the interactions between the various biological molecules.
Communication skills - Students' ability to talk, discuss and reflect on the topics raised during the lessons will be stimulated, especially insisting on the importance of the scientific method that led to the statements dealt with during the course.
Learning skills - Being able to discuss scientific issues concerning the interaction between molecules and the cellular and molecular system also in its biomedical applications and in the implications in the relationship of biochemistry with health. This skill will be developed and tested by involving students in oral classroom discussions.

Teacher's Profile

courseProgram

Proteins: amino acids, peptide bond. Primary structure. Secondary structure: alpha helix, beta structure. Protein fibrose: keratin, collagen, elastin. Tertiary structure: globular proteins, protein stability and folding. Quaternary structure. Myoglobin. Hemoglobin: structure, heme (synthesis and degradation), oxygen bond, bond cooperativity, 2,3-bisphosphoglycerate, allosteric regulation. Pathological hemoglobins.
Enzymes: introduction to enzymes: holoenzyme, apoenzyme, cofactors, classification, substrate specificity. Enzymatic kinetics, Michaelis-Menten equation, Km, Kcat and Kcat / Km. Enzymatic inhibition: competitive, non-competitive, mixed. Mechanism of action of serine proteases.
Carbohydrates: references to monosaccharides and disaccharides of biological interest. Interconversion of sugars. Structural polysaccharides (cellulose and chitin). Deposit polysaccharides (starch, glycogen). Krebs cycle. Glycoprotein: structure and function. Nucleotide formation. Blood groups.
Lipids: fatty acids, triglycerides, glycerophospholipids, sphingolipids, cholesterol, lipoproteins. Properties of lipid aggregates, micelles and liposomes and lipoproteins. Biological membranes: chemical composition, membrane structure. Movement of molecules through membranes, pores and channels. Active and passive transport simporto, antiporto etc.
Water-soluble vitamin: general, definition and relationship with coenzymatic activity. Fat-soluble vitamins (A; D; E; K). For each vitamin: chemical structure, any biochemical precursors, deficiency effects, biochemical mechanism of action.

examMode

The exam takes place in the forms established by art. 23 of the University Teaching Regulations. A specific report is drawn up for this purpose, signed by the President and by the members of the commission and by the student examined. The vote is expressed in thirtieths, with possible praise. Passing the exam requires the awarding of a grade not lower than eighteen / thirty and involves the assignment of the corresponding university educational credits. In the evaluation of the tests and in the attribution of the final grade, the following will be taken into account: the level of knowledge of the demonstrated contents (superficial, appropriate, precise and complete, complete and thorough), the ability to apply the theoretical concepts (errors in applying the concepts , discreet, good, well established), of the capacity for analysis, synthesis and interdisciplinary connections (sufficient, good, excellent), of the capacity of critical sense and of formulation of judgments (sufficient, good, excellent), of the mastery of expression (exposure lacking, simple, clear and correct, safe and correct).
In particular, questions will be asked about nutrients, their interaction with cells, organs and tissues and their effects on human health. Finally, knowledge on the digestion and absorption of nutrients and their metabolic fate will be requested.

books

1) Lecture notes.
2) I principi di biochimica di Lehninger di David L. Nelson, Michael M. Cox
Zanichelli Editore ISBN: 8808920690
In alternativa.
3) La Chimica Organica e le macromolecole biologiche, di Butera-Lauricella
Piccin Nuova Libraria ISBN:978-88-299-3057-9

mode

The Biochemistry course will take place through lectures in the classroom supported by slides and graphic and photographic illustrations. The teaching method used will tend to provide the tools with a critical view of the biochemistry sector through practical examples and the comparison with different animal and plant species. Frontal lessons are equivalent to 7 credits (48 hours).

classRoomMode

Course attendance is not mandatory, however it is strongly recommended.

bibliography

1) Lecture notes.
2) I principi di biochimica di Lehninger di David L. Nelson, Michael M. Cox
Zanichelli Editore ISBN: 8808920690
In alternativa.
3) La Chimica Organica e le macromolecole biologiche, di Butera-Lauricella
Piccin Nuova Libraria ISBN:978-88-299-3057-9

118397 - MOLECULAR BIOLOGY

SARA RINALDUCCI

Second Semester 7BIO/11ita

Learning objectives

Educational objectives
Course objective is to provide an overview of the molecular structure and function of the nucleic acids. The goal is to encourage students to acquire solid basic knowledge of molecular biology for studying and understanding the organization and evolution of genomes, but especially the molecular mechanisms that, in prokaryotes and eukaryotes, underlie and govern both the maintenance and the flow of genetic information. Moreover, the student will learn the basics of intracellular communication through the study of signal transduction pathways responsible for short-term responses.
Knowledge and understanding - To possess the basics of biochemistry underlying the structure of nucleic acids, for understanding its biological function. Having acquired the concepts and knowledge necessary to understand the molecular and cellular bases of replication, transcription and translation of the genetic material in prokaryotes and eukaryotes. To understand the main mechanisms of intracellular signaling and of functional protein-protein interactions.
Applying Knowledge and understanding - Knowing how to use the theoretical notions learned during the course for a critical analysis of molecular mechanisms at the basis of life.
Making judgements -To be able to discuss the role of the cellular processes described in class, increasing the ability to translate the acquired theoretical concepts into application.
Communication skills - To demonstrate the ability to summarize and effectively present the acquired information. To develop the ability to use the correct terminology.
Learning skills - To be able to grasp, rework and discuss the scientific issues dealt with in the lesson, including their evolutionary implications.

Teacher's Profile

courseProgram

NUCLEIC ACIDS. The DNA structure. The double helix and Watson-Crick base pairs (DNA B). Alternative DNA secondary structures (DNA A, DNA Z). DNA topology (supercoiling, topoisomerases). DNA denaturation and renaturation. The RNA structure. Spatial arrangements of RNA.
GENOME EVOLUTION AND ORGANIZATION. The gene: definition and structure in prokaryotes and eukaryotes. Unique and repetitive sequences of DNA, coding and noncoding regions. The nucleosome and chromatin organization. Chemical modifications of histone N-terminal tails and their functional significance. DNA methylation.
DNA REPLICATION. Structure and function of DNA polymerase. Fidelity and processivity of DNA polymerase. Specialized DNA polymerases. DNA helicase. The mechanism of DNA replication: initiation phase, strand synthesis, replication fork, termination, telomere and telomerase. Regulation of DNA replication in prokaryotes and eukaryotes.
TRANSCRIPTION IN PROKARYOTES. RNA polymerase. Bacterial promoter recognition. RNA biosynthesis and maturation: initiation, elongation and termination steps.
TRANSCRIPTION IN EUKARYOTES. Eukaryotic promoters. RNA polymerase I, II and III. Key differences in transcription in eukaryotes vs. prokaryotes.
PRINCIPLES OF TRANSCRIPTIONAL REGULATION. Repressors and activators. DNA-binding domains in transcriptional regulators. Examples of gene expression regulation in prokaryotes and eukaryotes.
RNA MATURATION. tRNA and rRNA maturation processes. Modifications of eukaryotic mRNA: capping, polyadenylation, editing. The splicing mechanism: chemistry of the reaction, spliceosome assembly.
TRANSLATION OF GENETIC INFORMATION. The mechanism of protein synthesis in prokaryotes and eukaryotes. Examples of translation regulation. Non sense-mediated and non stop decay (NMD, NSD).
PROTEIN POST-TRANSLATIONAL MODIFICATIONS. Lipidation, glycosylation, phosphorylation, acetylation, methylation, ubiquitination and sumoylation of proteins.
INTRACELLULAR SIGNALING. Steroid hormones. Single- and multipass transmembrane receptors. G-proteins, AMPc, PKA, inositol triphosphate, DAG and PKC. Calcium signaling.

examMode

The examination will consist of an interview with open questions (at least three) on the topics covered during the course. The acquisition of basic knowledge concerning processes that involve DNA as the genetic material, with particular reference to the structure of nucleic acids, the mechanisms of duplication, transcription, RNA modifications, protein synthesis, intracellular signaling. In addition, the capabilities of analysis, synthesis and self-contained inter- and interdisciplinary connections will be tested. For the exam assessment and the award of the final vote, the mastery of expression will also be taken into account.

books

BIOLOGIA MOLECOLARE di F. Amaldi, P. Benedetti, G. Pesole, P. Plevani (2018-terza ed. Casa Editrice Ambrosiana); BIOLOGIA MOLECOLARE: principi e tecniche di M.M. Cox, J.A. Doudna, M. O'Donnel (2013-Zanichelli). BIOLOGIA MOLECOLARE DELLA CELLULA di B. Alberts, A. Johnson, J. Lewis, D. Morgan, M. Raff, K. Roberts, P. Walter (2016-sesta ed. Zanichelli).

mode

The course consists of classroom lessons in which the topics covered by the program will be presented by using Power Point slides containing pictures and videos.

classRoomMode

Attendance at lessons is not compulsory, but strongly recommended

bibliography

The teaching material will be available on the Moodle platform. Non-attending students are encouraged to contact the professor for information on the program and on any additional bibliography.

120887 - GENERAL AND POPULATION ECOLOGY

ROBERTA BISCONTI

Second Semester 8BIO/07ITA

Learning objectives


Educational objectives
The course aims to provide solid basic knowledge of the structure and functioning of environmental systems, with particular emphasis on the mechanisms that determine the distribution and abundance of organisms as well as their relationships with the environment. The course aims to emphasize the hierarchical and interdisciplinary nature of ecology with the aim of encouraging students to deal with complex and multi-scalar disciplines and problems. This is reflected in the organization of the program, that runs the hierarchical scale of the ecological organization, from the mechanisms underlying biodiversity (evolutionary ecology), through the relationships between organisms and species (population ecology) to the structure and functioning of the communities (community ecology).
Knowledge and Understanding -By the end of the course, students will have gained fundamental knowledge of organism-environment relationships and evolutionary ecology, with a focus on population dynamics and interspecific interactions. This understanding will be developed through lectures and practical exercises.
Applying Knowledge and Understanding - Students will be able to apply the acquired knowledge to understand environmental issues, improving their ability to explain and discuss complex problems while managing information across multiple hierarchical levels. Additionally, students who complete the course successfully will strengthen their ability to use scientific methods to describe and explain the complex realities of the biosphere.

Making Judgements - By the end of the course, students will be able to apply critical review tools from the scientific literature to formulate interpretative hypotheses based on experimental data. This objective will be supported by field exercises, in which students will be driven to describe and compare different environmental contexts by collecting, processing, and analyzing data.
Communication Skills -Throughout the course, students will be encouraged to actively participate in discussions and share their opinions to enhance their communication abilities. By the course's conclusion, they should be proficient in clearly and effectively conveying the knowledge they have acquired.
Learning Skills - By the end of the course, students will be able to independently formulate well-supported, evidence-based scientific questions and design experimental approaches, integrating their knowledge with the existing scientific literature.

120363 - BIOLOGICAL EVOLUTION

Second Semester 6BIO/07ita

Learning objectives

TRAINING OBJECTIVES
The aim of the course is to deepen the understanding of the most important evolutionary theories, the mechanisms of evolutionary change, and the significance of both historical and contemporary evolutionary processes in generating and shaping the structure of biological diversity at all levels of organization. Knowledge will also be provided regarding the applications of principles and methods of evolutionary biology to areas such as the exploitation and management of natural resources, nature conservation, climate change biology, invasion biology, medicine, and agriculture.
EXPECTED LEARNING OUTCOMES
1. Knowledge and understanding: Understand how evolutionary processes operate and interact over time and space, influencing biodiversity and species adaptation.
2. Applied knowledge and understanding: (a) Apply methods and tools of evolutionary analysis to interpret biological data, such as phylogenetic analyses and population genetics studies; (b) Develop the ability to design and conduct experiments to test evolutionary hypotheses, analyzing and interpreting the results obtained.
3. Judgment autonomy: Knowledge and practical application will enable critical evaluation of different evolutionary theories and the scientific evidence supporting them.
4. Communication skills: Knowledge and practical application will also provide the necessary means to communicate clearly and effectively the concepts of evolutionary biology and the results of experiments to both specialized and non-specialized audiences, using appropriate communication methods, including scientific articles, oral presentations, and posters.
5. Learning ability: The ability to learn will be assessed through exercises and presentations of scientific work that demonstrate the level of understanding of the topics covered and the capacity to adapt and integrate new knowledge and emerging techniques in the field of evolutionary biology.

OPTIONAL GROUP - -- -
MODULO B ( APPLICATION) THEORY AND APPLICATION IN BIODIVERSITY CONSERVATION - BEGINNERS - BA

GIANLUCA PIOVESAN

Second Semester3BIO/07ENG
MODULO A ( THEORY ) THEORY AND APPLICATION IN BIODIVERSITY CONSERVATION - BEGINNERS - BA

GIANLUCA PIOVESAN

Second Semester3BIO/07ENG
SUBJECTSEMESTERCFUSSDLANGUAGE
118400 - GENERAL PHYSIOLOGY AND ECOPHYSIOLOGY

DAVID COSTANTINI

First Semester 9BIO/09ita

Learning objectives

Educational objectives
The course aims to provide solid knowledge on physiological processes, from the cellular to the organism level. In particular, the course will allow you to:
1) understand the functions and the mechanisms of the physiological processes at cellular, organismal, and system level and their regulation and functional integration through different levels of organization in living organisms;
2) understand the adaptive and homeostatic capacities of organisms in response to environmental changes, both social and physical.
The course will pose a solid basis for more specialized studies in neuroscience, behavioural physiology, comparative physiology, ecophysiology, and conservation physiology.
Knowledge and understanding - Physiological processes and mechanisms in animals, with ability to connect and integrate different systems and other biological disciplines.
Applying knowledge and understanding – Understand the physiological connections between systems, of the relationships between organism and environment (internal and external) mediated by physiological processes, including the basic knowledge of the experimental approaches, also pharmacological, for the study of physiological mechanisms with emphasis on the coping response to stress, environmental challenges, and the interaction between ecological and physiological processes.
Making judgments - Ability to interpret the results of physiological studies; to understand role of the physiological responses of the organisms to the social and non-social environment; being able to understand the evolutionary and functional connection between anatomy, physiology, behaviour and environment. Being aware of the animal diversity of mechanisms that control the activity of the organisms;
Communication skills - Ability to describe physiological issues, with the appropriate technical/scientific language, both written and spoken;
Learning skills - Ability to use the acquired knowledge for understanding the physiological processes and mechanisms, from the ionic and biochemical to the organismal level. The achievement of this goal will also be pursued with group discussions on specific topics, also suggested by the students.

Teacher's Profile

courseProgram

History of physiology; adaptation and phenotypic plasticity; experimental design; cell structure; cell membrane receptors; diffusion and osmosis; active transport; homeostasis; feedback concept; electrophysiology and action potential; energy and metabolism; oxidative stress; physiology of stress and glucocorticoids; principles of immunology; neuron; propagation of nerve impulses; synapses; central and peripheral nervous system; autonomic nervous system; muscle physiology; cardiovascular system; respiratory system; digestive system and nutrition; endocrine system (glands and hormones); thermal relationships; gas exchanges; osmoregulation; physiological regulation of migratory behaviour; orientation; physiology of reproduction; physiology and life-history; principles of ecophysiology and conservation physiology (captive breeding programs, land-use change, global warming); physiological consequences of exposure to chemical and non-chemical pollutants (noise and light pollution); physiology of growth and development; physiological basis of sexual and social signals.

examMode

The assessment test is oral and will contain a series of questions aimed at ascertaining the student's theoretical knowledge on the interpretation of the physiological mechanisms regulating adaptations of organisms to their environments. In addition, some questions will be aimed at solving a practical problem on the type of those faced during the lessons. The methods for attributing the final judgment are based on the number of correct answers, which must be greater than 60% of those proposed.

books

In italian:
POLI et al., FISIOLOGIA ANIMALE, EdiSES
STANFIELD, FISIOLOGIA, EdiSES

In english:
HILL et al., ANIMAL PHYSIOLOGY, Sinauer Associates
WILLMER et al., ENVIRONMENTAL PHYSIOLOGY OF ANIMALS, Wiley-Blackwell
BUTLER et al., ANIMAL PHYSIOLOGY, AN ENVIRONMENTAL PERSPECTIVE, OUP Oxford
MOYES and SCHULTE, PRINCIPLES OF ANIMAL PHYSIOLOGY, Pearson

mode

The course includes lectures, interactive and supported by Power Point presentations, audiovisuals, with stimulation to deepen specific topics and the choice of potential thesis topics. Propose an understanding of the multidisciplinary, interdisciplinary and integrative nature of the topics covered. Case study discussion. Training seminars on specific topics. Critical discussion of scientific articles.

classRoomMode

Attendance is not compulsory but highly recommended.

bibliography

Romero 2004. Physiological stress in ecology: lessons from biomedical research. Trends in Ecology and Evolution, 19, pp. 249-255.
Cooke et al. 2013. What is conservation physiology? Perspectives on an increasingly integrated and essential science. Conservation Physiology, 1, 10.1093/conphys/cot001.
Costantini 2019. Understanding diversity in oxidative status and oxidative stress: the opportunities and challenges ahead. Journal of Experimental Biology, 222, jeb194688.

118402 - APPLIED ECOLOGY

ALEIDA ASCENZI

First Semester 7BIO/07ita

Learning objectives

Educational objectives
The objectives of the course are the transmission of the basic notions of applied ecology useful for correctly framing the environmental themes deriving from the interaction between man and natural ecosystems. The course introduces the main characteristics of ecosystems and analyzes the impacts on these ecosystems deriving from human activities, providing for each theme an overview of the management, technical and regulatory tools used to stem environmental degradation.
Knowledge and understanding - At the end of the training the student will acquire the knowledge concerning the main anthropic impacts on marine, terrestrial and inland water ecosystems. The student will be able to understand the multiple effects that human activities imply, in terms of the imbalance of the stability of natural ecosystems.
Applying knowledge and understanding - The student will be able to find out about the technical options that can be applied in solving the most pressing environmental issues and will be able to identify the national and supranational regulatory tools through which these issues can be addressed.
Making judgments - During lectures and exercises, open discussion on the main environmental issues addressed is encouraged, in particular with regard to possible solutions to the most current environmental emergencies. The student will therefore acquire autonomy of judgment based on the knowledge learned and the dialectical comparison with colleagues and the teacher.
Communication skills -The student will acquire an appropriate technical-scientific language during the lessons and exercises, through the knowledge of the definitions and the main basic concepts in ecology and applied ecology. Communication skills will be verified during the exam.
Learning skills - The student will have to demonstrate to be able to use the concepts learned to hypothesize the possible effects of human activities even in environmental contexts different from those considered as an example, based on the ecological characteristics of the considered environment.

118384 - ENVIRONMENTAL BIOLOGICAL MONITEERING LABORATORY - 12- -

Learning objectives

ENVIRONMENTAL BIOLOGICAL MONITORING LABORATORY - Module I
Educational objectives
The objectives of the course are the transmission of the basic notions of ecotoxicology useful for correctly framing the issues relating to environmental and human health. The course introduces environmental issues related to the presence of xenobiotic chemicals and focuses attention on the mechanisms of cellular toxicity. The processes that lead to persistence / degradation, bioconcentration, bioaccumulation, biomagnification, the use of cellular biomarkers and the use of in vitro cell cultures as a useful tool in the study of toxicity at various levels are the central themes of the course. The laboratory exercises aim to learn the main techniques for the in vitro cultivation of mammalian and / or fish cell lines and the procedures necessary to perform cytotoxicity and / or genotoxicity tests.
Knowledge and understanding - At the end of the training activity the student will acquire knowledge on what are the chemical / physical characteristics of the contaminants and the biotic and abiotic processes capable of influencing their environmental path and toxicity, will be able to describe the general principles to evaluate the direct and indirect effects of different environmental contaminants on various levels of biological organization, research, evaluate, process relevant ecotoxicological information. Will be able to understand, organize and present data relating to an ecotoxicological study.
Applying knowledge and understanding - At the end of the training, the student will have to demonstrate that they are able to experimentally manipulate cells in vitro to be used for ecotoxicological tests.
Making judgments - During the exercises and the bibliographic study, the student will acquire autonomy of critical judgment on the basis of the knowledge learned for the assessment of environmental risk and possible remedies.
Communication skills -The student will acquire an appropriate technical language while studying articles provided in class. The methods learned during the exercises include a short practical test. Communication and exhibition skills will be verified during the exam.
Learning skills - The student will have to demonstrate to be able to use the methods learned to investigate different ecotoxicological problems. The student must be able to critically relate an ecotoxicological research through a presentation in ppt

ENVIRONMENTAL BIOLOGICAL MONITORING LABORATORY - Module II
Educational objectives
The course aims to develop skills in the design and execution of biological monitoring according to the principles and methods contained in current European and Italian legislation.
Knowledge and understanding -The knowledge acquired will concern the general principles on which biological monitoring activities are based, the methodological processes for the implementation of monitoring campaigns and the determination of biological indices of environmental quality.
Applying knowledge and understanding -The application of knowledge will be focused on the methodological study concerning the indices currently in use to evaluate the ecological status of the river courses.
Making judgments -The ability to formulate independent evaluations can be exercised in the interpretation of the results, coordinating the heterogeneous data to arrive at reasoned judgments.
Communication skills -These skills will be developed through the exercise of one's own expression (interventions during the lessons) and in the coordination of group activities.
Learning skills -The methodological study can allow the extension of the concepts and practices to the monitoring application to other environmental matrices.

ENVIRONMENTAL BIOLOGICAL MONITEERING LABORATORY (MODULE I)

DANIELA WILLEMS

6BIO/06ita

Learning objectives

ENVIRONMENTAL BIOLOGICAL MONITORING LABORATORY - Module I
Educational objectives
The objectives of the course are the transmission of the basic notions of ecotoxicology useful for correctly framing the issues relating to environmental and human health. The course introduces environmental issues related to the presence of xenobiotic chemicals and focuses attention on the mechanisms of cellular toxicity. The processes that lead to persistence / degradation, bioconcentration, bioaccumulation, biomagnification, the use of cellular biomarkers and the use of in vitro cell cultures as a useful tool in the study of toxicity at various levels are the central themes of the course. The laboratory exercises aim to learn the main techniques for the in vitro cultivation of mammalian and / or fish cell lines and the procedures necessary to perform cytotoxicity and / or genotoxicity tests.
Knowledge and understanding - At the end of the training activity the student will acquire knowledge on what are the chemical / physical characteristics of the contaminants and the biotic and abiotic processes capable of influencing their environmental path and toxicity, will be able to describe the general principles to evaluate the direct and indirect effects of different environmental contaminants on various levels of biological organization, research, evaluate, process relevant ecotoxicological information. Will be able to understand, organize and present data relating to an ecotoxicological study.
Applying knowledge and understanding - At the end of the training, the student will have to demonstrate that they are able to experimentally manipulate cells in vitro to be used for ecotoxicological tests.
Making judgments - During the exercises and the bibliographic study, the student will acquire autonomy of critical judgment on the basis of the knowledge learned for the assessment of environmental risk and possible remedies.
Communication skills -The student will acquire an appropriate technical language while studying articles provided in class. The methods learned during the exercises include a short practical test. Communication and exhibition skills will be verified during the exam.
Learning skills - The student will have to demonstrate to be able to use the methods learned to investigate different ecotoxicological problems. The student must be able to critically relate an ecotoxicological research through a presentation in ppt

Teacher's Profile

courseProgram

Ecotoxicology as a multidisciplinary approach. The importance of environmental toxicology and cellular toxicology.
Reviews of cellular structure and function and the organization of livings (cells, tissues, organs, and organisms). Cellular and molecular toxicity.
The environment as an agent capable of modifying phenotype and influencing molecular aspects of development. Environmental interferences in regulating gene transcription . Transmission of epigenetic memory between generations. Epigenetic transmission of xenobiotic damage. The importance of ontogeny for adult health and disease.
Developmental toxicology. Environmental compartments, environmental fate of a pollutant. Air, water, and soil. Impact of pollutants on the environment and human health.
The REACH program and Directive 91/414/EEC on the market of chemicals. Main classes of synthetic inorganic and organic pollutants and evidence of their environmental effects. Historical notes on environmental contamination. The main physicochemical properties of a pollutant , environmental persistence, degradation pathways in nature.
Persistent pollutants (POPs) and global contamination. Bioaccumulation, biomagnification. Toxic effects of metals. Biotransformation of xenobiotics. Target organ toxicity.
The primary target of pollutants. The main biomarkers. The relationship between individual response and ecological effects.
The study of effects: exposure, absorption, distribution, and excretion. Evaluation of effects: toxicological tests at different levels of organization and complexity (laboratory, mesocosms, etc.).
Toxicity tests. Use of bacteria, algae, crustaceans, fish, and mammals,
Assessment tests (acute and chronic) and endpoints (LC50, EC50, IC50, NOEC, LOEL). In vitro cell cultures applied to ecotoxicological studies, according to the REACH regulation.
Notes on predictive models for assessing the environmental fate of organic pollutants. QSAR (Quantitative Structure Activity Relationship) models. The congeneric nature of molecules. Mixtures of toxic substances in the environment: an approach to the problem. Some molecular descriptors.
Organ, tissue, and cell culture. Plant and animal cell culture.
Culture techniques, good laboratory practice, maintaining sterile conditions, contamination, prevention of contamination,culture media, and antibiotic use. Cell growth, parameters, confluence, and cell detachment.

examMode

Practical test on the techniques acquired in laboratory practice and oral discussion of a scientific article

books

Fundamental of Ecotoxicology: the Science of Pollution-- Newman M.C.
Ecological Developmental Biology-The Environmental Regulation of Development, Health, and Evolution -
Gilbert S.F.,Epel D.

classRoomMode

Mandatory laboratory lessons

ENVIRONMENTAL BIOLOGICAL MONITEERING LABORATORY (MODULE II)

ADRIANA BELLATI

6BIO/05ita

Learning objectives



ENVIRONMENTAL BIOLOGICAL MONITORING LABORATORY - Module II
Educational objectives
The course aims to develop skills in the design and execution of biological monitoring according to the principles and methods contained in current European and Italian legislation.
Knowledge and understanding -The knowledge acquired will concern the general principles on which biological monitoring activities are based, the methodological processes for the implementation of monitoring campaigns and the determination of biological indices of environmental quality.
Applying knowledge and understanding -The application of knowledge will be focused on the methodological study concerning the indices currently in use to evaluate the ecological status of the river courses.
Making judgments -The ability to formulate independent evaluations can be exercised in the interpretation of the results, coordinating the heterogeneous data to arrive at reasoned judgments.
Communication skills -These skills will be developed through the exercise of one's own expression (interventions during the lessons) and in the coordination of group activities.
Learning skills -The methodological study can allow the extension of the concepts and practices to the monitoring application to other environmental matrices.

Teacher's Profile

courseProgram

Changes in environmental quality, pollution, bioindicators and environmental monitoring;
Sampling techniques, including complex environmental matrices, collection tools and biological conservation;
Biomonitoring applications for assessing air quality (IBL index, O3 biomonitoring);
Biomonitoring applications for assessing soil quality (QBS index);
Biological monitoring of surface water bodies;
General characteristics of inland waters: lentic and lotic environments (structure of lakes and self-purifying capacity, metabolic efficiency, river continuum concept, ecological and hydroclimatic factors of rivers);
The use of biological indicators for assessing the quality of running waters;
The biological indices (of diversity, the saprobic system and the biotic indexes BMWP, IBE, ASPT);
The European Water Framework Directive (2000/60 / EC): typing, different types of monitoring, reference conditions;
The elements of biological quality (EQB): Macroinvertebrates, Macrophytes, Diatoms and Pisces;
The Macroper index;
The assessement of the ecological status of a superficial watercourse through Macroinvertebrates and the elements of chemical-physical and hydromorphological quality in support.
The most recent methodologies for the isolation and the analysis of diagnostic DNA markers for environmental monitoring.

examMode

Oral exam on principles and general concepts of environmental biomonitoring.
Focus on Water Framework Directive.
Practical test of recognition of benthic macroinvertebrates.
Application of the STAR_ICMi.

In order to passa the exam, the student must be confident with the general principles of biomonitoring. The final mark will be formulate according to the familiarity of the student with concepts and principles of biomonitoring and he/she ability in describing protocols for biomonitoring.

books

Water Framework Directive (2000/60/CE)
IRSA manuals
ISPRA manuals
Atlases and identification cards
The textbooks will be provided by the teacher, who will indicate the links for the retrieval

mode

Lectures (20 hours, blended modality), field and laboratory exercises (8 hours, sampling and separation of organisms, taxonomic identification), classroom exercises (12 hours, application of monitoring indices).

classRoomMode

Attending lessons is not mandatory. Attending fieldwork, laboratory activities and classroom excercises is not mandatory as well, but strongly encouraged.

bibliography

See Texts

118401 - GENERAL AND ENVIRONMENTAL MICROBIOLOGY

SUSANNA GORRASI

Second Semester 9BIO/19ita

Learning objectives

Formation Goals.
The course will permit the comprehension of the morphological, physiological and metabolic characteristic of microorganisms (mainly prokaryotic); their role in nature and the interactions with other organisms. Moreover, it will consent to acquire the basic techniques for the manipulation of microorganisms in laboratory, their identification and the study of microbial communities. The course will permit to understand the principal interactions between microorganisms and environment particularly in relation with the bio-geochemical cycles. The course will supply a reference framework to comprehend modern aspect related to the microbial world and the potential applications of useful microorganisms. The course will supply the basic formation for further specialized studies in microbial ecology, environmental and medical microbiology.
Learning results
Knowledge and understanding (ability). To get the knowledge regarding the basic principles of the microbial biology, their metabolism, their role in nature, the interaction between microorganisms environment and other organisms, their potential in biotechnology.
Applying knowledge and understanding (Ability to apply knowledge and understanding). To understand the issues related to the relationships between microorganism, mankind and environment. To acquire the basic knowledge regarding the experimental approach to study the microbial world.
Making judgement (Independent judgement). The ability to construe the results obtained by the various studies in microbiology. The ability to understand the role of the microorganisms in the environment and their influence on the biology of other organisms.
Communication skills. Ability to describe scientific topics related to microbiology both in written and oral forms by a specific scientific/technical language.
Learning skills. Ability to use the methods acquired for microorganism recognition and cultivation. This ability will be developed also trough the active involvement of the students in discussions during the lectures and by laboratory experiences.

Teacher's Profile

courseProgram

1) History of microbiology: discovery of the microbial world; the “Abiogeny” dispute, microorganisms and their environment, impact of microorganisms on man: pathogenic, harmful and useful microorganisms.
2) The laboratory of microbiology: the microscope, mentions of optical physics, preparation of microscope slides, electron microscopy and other microscopy techniques. The pure culture, microbial nutrition and preparation of culture media. Theory and practice of sterilization.
3) Cytology: prokaryotic and eukaryotic cells: prokaryotic and eukaryotic cells: general concepts, structure and ultra-structure of bacterial cells, structure and function of cytoplasmic membrane, transports through the cytoplasmic membrane, cell wall, structure and function, the cell wall of Gram + and Gram – bacteria, Archaeal and eukaryotic cell walls, capsules and other envelopes, motility, flagella and chemotaxis, endospore structure and function, mentions of eukaryotic spores.
4) Cell physiology: reminders of chemistry and cell biochemistry (redox reactions, hydrogen and electron transport, high energy compounds), biological energy production, glycolysis and alternative pathways; NAD re-oxidation: fermentations (alcoholic and lactic); aerobic respiration (TCA, electron transport phosphorylation, energy balance in respiration), mentions of anaerobic respiration and biosynthesis.
5) Microbial growth: growth of a single cell and of a microbial population, diauxic growth, effect of environmental parameters on growth (pH, temperature, etc.), methods for the control of microbial growth.
6) Principles of molecular and bacterial genetics (reminders): DNA structure, restriction enzymes, DNA replication, genetic elements, gene rearrangement, transposons, transcription, structure and function of mRNA and tRNA, translation and protein synthesis, genetic code mutations and mutagens; bacterial recombination (in details), transformation, transduction, plasmids and conjugation.
7) Virology: “anatomy” and structure of viral particles; viral counts; general concepts of viral reproduction, principles of viral genetics, RNA/DNA phages, temperate and lytic bacterial viruses; some important animal and plant viruses (mentions).
8) Microbial ecology: isolation and identification of microorganisms (recalls), interactions among microbial population and between microorganisms and other organisms; microbial ecosystems; methods for the study of microbial ecology and diversity; biogeochemical cycles (Carbon, Nitrogen, Iron, Sulphur, etc.).
9) Microbial classification and phylogeny (mentions)
10) Environmental biotechnology: pollution and depollution; role of microorganisms in environmental decontamination, Soil and water bioremediation; aerobic/anaerobic catabolism of environmental pollutants, treatment of water and wastewater; Some study cases.
11)Microbial Biotechnology: microorganism of industrial interest, screening for industrial metabolites/enzymes. Bioreactors, structure and design, scale-up, primary and secondary metabolites; production of antibiotics, enzymes, etc. (mentions). Enzyme and cell immobilization (mentions)
12) Pathogenic, harmful and useful microorganisms, mentions of medical microbiology. Pathogenic microorganisms in food and waters (mentions); Microbiological analysis of water

Some practical/laboratory lectures will be added to better explain some topics of laboratory techniques

examMode

The examination goal is to verify the general level of comprehension of the discipline. The student evaluation will be done by an oral examination regarding the whole course program. The examination will be considered approved by a minimum score of 18/30.

books

Brock, Biologia dei Microrganismi di M.T. Madigan e J.M. Martinko, D.A. Stahl, D.P. Clark, Pearson, 2012. Vol.1 e 2 (or any other more recent edition)
Brock, Biologia dei Microrganismi di M.T. Madigan e J.M. Martinko, Casa Editrice Ambrosiana. Vol. 1 e 2A
Biologia dei microrgamismi di G. Dehò e E. Galli, Casa Editrice Ambrosiana, 2018.
Microbiologia ambientale ed elementi di ecologia microbica di Barbieri, Bestetti, Galli, Zannoni, Casa Editrice Ambrosiana, 2008

The lectures slides (PDF) are available on line. During the course, some scientific publications will be distributed and discussed.

Other recent textbooks of General Microbiology could be used after a preliminary check with the teacher.

mode

The course will consist in oral lectures regarding the published program and supported by Power Point presentations (available on line on Moodle platform). It is possible that few scientific papers will be discussed too

classRoomMode

Attendance to classes is not mandatory, but it strongly suggested to better understand the topics

bibliography

The suggested text books are sufficient to get the necessary information. The teacher will supply additional literature if necessary.

118403 - BIOLOGICAL OCEANOGRAPHY

MARCO MARCELLI

Second Semester 8BIO/07ita

Learning objectives

Educational objectives
The sea and oceans are home to fundamental biological and physical processes for the life and physical and chemical balance of the entire planet and are rich in renewable living resources, which provide valuable nutrients and molecules for human life and health. They are also such a favorable habitat for life as to constitute a privileged location for the development of life and have a very high biodiversity, with all the Phyla represented, of which many are exclusively marine. The aim of the course is therefore to build an educational path that, through lectures, laboratory and sea exercises, leads students to know the habitats, the functioning mechanisms of the marine ecosystem in its abiotic and biotic components, analyzing their interrelationships at different spatial and temporal scales.
Knowledge and understanding -To have developed knowledge related to the physical, chemical and biological properties of sea water. Knowledge related to the main marine, oceanic and coastal habitats and to pelagic and benthic organisms. Understanding of the main mechanisms of functioning of the marine ecosystem in its abiotic and biotic components by analyzing their interrelationships at different spatial and temporal scales.
Applying knowledge and understanding - Experimental approach to the study of oceanography and to the study of marine ecosystems through the use of the main sampling techniques and analysis methodologies presented during the lectures and carried out during the didactic exercises.
Making judgments - Being able to evaluate and deal with the appropriate skills, competences and critical sense of the study of the different issues concerning the oceanic and coastal marine environment presented during the course.
Communication skills -Being able to master the main topics addressed during the course with a correct scientific language.
Learning skills - Being able to apply the knowledge acquired, during lectures and exercises, to cases that require the integration of the topics covered during the course.

Teacher's Profile

courseProgram

The course includes part of theoretical lessons and exercises in the laboratory and in the field, dealing with the topics specified below.
Historical notes on the birth of oceanography as a science.
Physiographic characteristics of marine environments. Origin and evolution of marine and oceanic waters. The water cycle and the oceans. The chemical composition of sea water, the interaction with the atmosphere, carbon dioxide and the balance of carbonates. The cyclic salts.
The physical properties of sea water. The Earth and the solar system. The energy balance of the Earth. The atmosphere and atmospheric circulation. Relations between climate and oceans. Exchanges of energy between the sea and the atmosphere. Energy distribution on the earth's surface. The distribution of the fundamental variables of marine and oceanic water masses. The Coriolis force. Horizontal and vertical movements of water masses, wave motion, sea currents and other dynamic phenomena.
Pelagic and benthic organisms. The ecotypological classification of marine organisms. Introduction to marine biology: phytoplankton, zooplankton, necton and benthos. The regulating variables of biological processes and the distribution of organisms. Phytoplankton and primary production. Solar radiation and its ecological significance. Biomass, production and biological productivity. Photosynthesis, production and growth. The environmental regulatory factors of PP. The limiting factors. Gross and net production. Trophic levels, food chains and webs. Trophic transfer efficiencies. Methods of estimating Primary Production at sea.
Marine, oceanic and coastal habitats. The zoning of the benthos and the Mediterranean benthic biocoenoses: the classifications of Perès Picard and Riedl.
Energy flows and biogeochemical cycles (nitrogen, phosphorus, carbon, silicon cycles). Energy flow and trophic structure of ecosystems: ecological pyramids and efficiencies.
The dynamics of marine ecosystems. Time and dimensional scales. Micro-scale processes: the boundary layer, the vertical structure and the biology of the mixed layer. Meso-scale processes: ecological phenomena associated with upwelling, fronts, tides and internal waves. Macro-scale processes: the ecology of large-scale phenomena (major currents, gyres, eddies). Oceans and Global Change: physical and biological aspects, the role of the "biological pump".

examMode

Written and Oral Exam

The purpose of the written test is a preliminary assessment preparatory to the oral exam.
The oral exam will have as its objective the verification of the knowledge and skills acquired during the course through the adequate exposure and argumentation of the topics addressed in the program and of the learning objectives.

books

Lecture notes by prof. Marco Marcelli, available on Unitus Moodle (https://moodle.unitus.it).

mode

Frontal lessons
Experimental activities
Seminars
Field didactic experiences
Organized external visits

classRoomMode

Optional attendance

bibliography

Mann, Kenneth Henry, and John RN Lazier. Dynamics of marine ecosystems: biological-physical interactions in the oceans. John Wiley
& Sons, 2013.
TRUJILLO, A. P.; THURMAN, H. V. Introductory Oceanography.
2007.
Marco Marcelli Lecture Notes

118404 - STAGE

Second Semester 6ita
118405 - THESIS

Second Semester 7ita

Learning objectives

Educational objectives
The course of molecular methodologies applied to environmental research aims to provide students with an overview of the biomolecular and analytical instrumental techniques used for environmental studies. Knowledge will be given to develop the knowledge of environmental influences on the biology of living organisms and on humans. Students will acquire conceptual tools to understand the methods for the study of the effects of pollutants on human health and the environment and for the evaluation of toxicological risk.
Knowledge and understanding - At the end of the course the students will have to know the modern techniques of molecular biology used in the environmental field. They will understand the principles underlying the main molecular and analytical methods for the study of environmental pollutants. They will be able to correctly use the appropriate terminology used in molecular and analytical instrumental methodologies. They will be able to describe the structural elements of the main tools of a biochemical / molecular laboratory (liquid chromatography, spectrophotometer, mass spectrometer). They will know the classification of environmental toxic chemicals and their effect on humans.
Applying knowledge and understanding - Mastery of analytical and molecular methods for the study of genetic-environmental variability. Ability to identify the analytical-molecular techniques to be applied in different cases. Ability to apply techniques related to the identification of environmental pollutants.
Making judgments - To have a concrete ability to integrate basic biomolecular knowledge and manage the complexity of problems in the environmental application field.
Communication skills - Ability to expose the acquired knowledge. The student must be able to present logically, concisely and rigorously, in various forms and with different tools, objectives, concepts, data and procedures of work or experimental analysis.
Learning skills - Develop autonomous learning skills in the field of the discipline in question to increase their knowledge, constantly updating and keeping informed about new developments and bio-molecular methods used in the environmental field.

Teacher's Profile

courseProgram

Exposure to environmental contaminants (Environmental pollution, Air pollution, Water pollution, Soil pollution ). Pollutants in the environment: origin, diffusion, accumulation. Classification of toxic chemicals of environmental relevance. Toxic heavy metals Toxic organic compounds (dioxins, furans and PCBs Polycyclic aromatic hydrocarbons). Toxicity of environmental contaminants towards cellular components, hints of genotoxicity. Genes and tumors, heredity of cancer risk. Molecular aspects of tumorigenesis. Cell death, apoptosis and necrosis in response to genotoxic agents. Molecular methods for studying the environment. PCR, agarose gel electrophoresis, cloning, sequencing. PCR in real time. Application of molecular techniques for the study and isolation of environmental DNA, DNA/ RNA Stable isotopic probe. Fluorescent hybridization in situ (FISH, CARD-FISH, Raman-FISH, Nano-SIMS). Microarray.
Analytical methodologies for the determination of all classes of environmental pollutants: liquid chromatography, gas chromatography, mass spectrometry for the quantitative and qualitative analysis of environmental pollutants.
The techniques -omics : metabolomics. of proteomic mass spectrometry coupled to database analysis.
Journal club with literature reading in the environmental field and use of molecular methodologies

examMode

The purpose of the oral test is to verify the theoretical basis of molecular biological methodologies relevant to the study of the environment.
The evaluation of the examination will particularly take into account the articulation, precision and significance of the acquired theoretical content treated in an appropriate language.

books

- Francesco Amaldi Piero Benedetti Graziano Pesole Paolo Plevani
Tecniche e metodi per la biologia molecolare
- Mauro Maccarrone: Metodologie biochimiche e biomolecolari
Strumenti e tecniche per il laboratorio del nuovo millennio

Teacher's notes and teaching materials are provided during classes.
At the beginning of the course, the teacher will provide a link to download the teaching material.

mode

Teaching is proposed through frontal lessons (48 hours). Learning materials will be made available to students prior to each lesson. The space will be dedicated to the analysis of scientific papers or normative texts which apply molecular methodologies in the field of the environment.

classRoomMode

The teacher recommends student involvement with shared discussion and analysis of the subjects presented. Students are encouraged to engage with the teacher throughout the lesson. Non-regular students are required to study the texts indicated in the bibliography and may consult the material produced by the teacher, such as the students present. All students requesting clarification should contact the teacher to make an appointment.

bibliography

The teaching material will be available on the Moodle platform. Non-attending students are encouraged to contact the professor for information on the program and on any additional bibliography.

Learning objectives

Educational objectives
The aim of the course is to provide students with the useful information to learn the theoretical bases of classical and modern hydrobiology and the theoretical and experimental approaches that have enabled their development today. Expanding knowledge on aquatic organisms from a trophic-functional point of view. Students will have to learn the logic of ecosystem analysis of aquatic environments and the methodologies suitable for evaluating structures and functions of ecosystems in aquatic environments. Notions will be provided to understand the most recent developments in the ecosystem of aquatic environments (food web theory, niche theory, network analysis), and an understanding of the importance of managing these environments will be stimulated. The course aims to provide the basis for further in-depth studies in the fields of inland water ecology and community ecology.
Knowledge and understanding - Having developed knowledge of the principles and ecological laws that underlie the ecosystem functioning of aquatic environments. Having acquired the notions of hydrobiology useful for undertaking ecosystem analyzes of the various environmental types of inland waters. Ability to
Applying Knowledge and understanding - Knowing how to use the notions learned in class and developed in the exercises to interpret the structures and functions of the various aquatic organisms and to solve problems in the various fields of hydrobiology. Judgment autonomy. Being able to identify the theoretical and experimental paths to be applied to the resolution of new problems even if similar to those discussed in class.
Communication skills - Students' ability to talk, reason and discuss the questions raised during the lessons on the topics will be stimulated.
Learning skills - Being able to discuss scientific issues related to hydrobiology and in general the academic ecology of aquatic environments also in its management applications and in its theoretical and experimental implications. This skill will be developed and tested by involving students in classroom discussions.

Teacher's Profile

courseProgram

Introduction to the aquatic environment: Chemical and physical properties of water, Water cycle, movement of heat, light and nutrients in water.

Freshwater environments and their characterisation: River environments. Lacustrine environments. Transitional aquatic ecosystems. Wetlands. Physiography and conservation issues. Other water environments (rivers and underground lakes, high altitude springs, hydrothermal springs, interstitial waters). Semi-natural and artificial environments.

Fauna and flora of inland waters: Overview of evolution and adaptations to the water medium and the different chemical-physical characteristics of water. Trophic-functional classification of aquatic organisms. Microorganisms, algae, fungi and plants. Protozoa. Invertebrates (Cnidarians, Bryozoans, Platelminths, Nematodes, Annelids, Crustaceans, Insects). Vertebrates (Amphibians, Reptiles, Mammals, Birds, Bony and Cartilaginous Fish)

Ecology of inland waters: Interactions between species. Community and metacommunity ecology and aquatic trophic networks. Theory and case studies. Elements of sampling techniques.

Conservation, management and monitoring of freshwater environments: Threats to inland water systems. Bioindication and ecological indices. Monitoring and management.

examMode

The examination is conducted in the form laid down in Article 23 of the University Teaching Regulations. Minutes are taken of its proceedings, signed by the chairman and members of the board and by the student examined. The grade is expressed in thirtieths, with honours where appropriate. Passing the exam requires a mark of no less than eighteen/thirty-eighths and entails the award of the corresponding university credits. In assessing the test and in awarding the final grade, account will be taken of: the level of content knowledge demonstrated (superficial, appropriate, precise and complete, complete and thorough), the ability to apply theoretical concepts (errors in applying concepts, discrete, good, well established), the ability to analyse, summarise and make interdisciplinary connections (sufficient, good, excellent), the ability to make a critical sense and formulate judgements (sufficient, good, excellent), the mastery of expression (poor, simple, clear and correct, safe and correct exposition). The question will concern concepts and base knowldge addressed during the lessons, with the free choice to present a study case (e.g. a scientific paper) regarding freshwater ecology themes, from which the examination discussion can start.

books

Walter, K., Dodds, W. H. I. L. E. S., & Matt, R. (2017). Freshwater ecology: concepts and environmental applications of limnology. ELSEVIER ACADEMIC Press.

ORNERIS G., PASCALE M., PEROSINO G.C., ZACCARA P. (2013). Lezioni di Idrobiologia Editore: Regione Piemonte, Direzione Agricoltura - settore caccia e pesca (https://greencrestdotblog.files.wordpress.com/2018/01/2008-lezioni-di-idrobiologia-le-acque-continentali.pdf)

classRoomMode

Attendance is optional, but students are strongly encouraged to attend the lectures; I will explain methods of researching scientific bibliography, which are very useful for the final examination project (optional), and useful in general for a scientific career. In addition, class discussions on course topics useful for further study will be stimulated.

Learning objectives

Educational objectives
The objectives of the course are transmission of the basics of geology useful to properly frame the environmental themes. The course introduces the composition of the Earth and focuses attention on the lithogenetic cycle. The exogenous and endogenous processes that give rise to formation of rocks and their structure constitute central themes of the course.
Knowledge and understanding - At the end of the training activity, the student will acquire knowledge about the inner constitution of the Earth and the main endogenous and exogenous processes that characterize the Planet. The student will be able to distinguish the most common types of rocks by linking them to their origins. The student will be able to understand the principles of stratigraphy and the main geological structures.
Applying knowledge and understanding - At the end of the training activity, the student will have to demonstrate that he is able: to distinguish and classify the main types of rocks, to read and interpret geological map.
Making judgments -Through the exercises on geological maps, the student will acquire autonomy of judgment based on the acquired knowledge, analyzing different Italian areas.
Communication skills -The student will acquire an appropriate technical language during the lessons and exercises on rocks classification and geological maps. Communication skills will be verified during examination.
Learning skills - The student must demonstrate his ability to investigate geological areas which differ from those considered during the course. The student must be able to analyze the essential geological features of an area.

Teacher's Profile

courseProgram

Shape and dimensions of the Earth. The internal structure of the Earth: core, mantle and crust. Lithosphere and asthenosphere. Notes on plate tectonics.
Elements of mineralogy: main physical and chemical properties, main minerals of rocks.
Magmatic processes and igneous rocks. Magmatic consolidation. Structure and texture of igneous rocks. Methods of classification of intrusive, extrusive and hypabyssal rocks. Characteristics of the main igneous rocks. Volcanism: types of eruptions and types of volcanoes. Pyroclastic rocks.
Exogenous processes and sedimentary rocks. Sedimentary processes: weathering, transport, sedimentation and diagenesis. Classification and characteristics of sedimentary rocks: silicoclastic; carbonatic; evaporitic; other chemical and biochemical rocks, residual rocks. Properties of sedimentary rocks. Principles of stratigraphy.
Metamorphic processes and metamorphic rocks. Metamorphic factors: temperature, pressure and fluid phase. Texture and structure of metamorphic rocks. Metamorphic facies and types of metamorphism. Main metamorphic rocks.
Outline of structural geology: folds, faults and thrusts.
Earthquakes: causes, mechanisms and distribution of earthquakes.
Overview of the chronology of Earth.
Topographical maps. Geological maps.
Erosive processes, rivers and river erosion, wind erosion, glacial erosion, coastal erosion, landslides.

examMode

The assessment consists of an oral test and takes place in about 30 minutes. The exam topics are those covered during the course. In particular, classification of rocks, principles of stratigraphy and structural geology, exogenous and endogenous processes, topographic and geological maps will be the exam topics. The test will also assess the candidate’s ability to apply knowledge to environmental contexts, technical language and scientific curiosity. The exam evaluation will be based on the oral exams results, with a score awarded out of thirty according to the "Regolamento didattico di Ateneo".

books

Grotzinger J.P., Jordan T.H. (2016) Capire la Terra. Zanichelli, Bologna.

classRoomMode

The attendance is not mandatory but is highly recommended.

CHOICE GROUPSYEAR/SEMESTERCFUSSDLANGUAGE
OPTIONAL GROUP -6 - -
118425 - MOLECULAR METHODOLOGIES APPLIED TO ENVIRONMENT

GIUSEPPINA FANELLI

Second Year / First Semester 6BIO/11ita
118393 - HYDROBIOLOGY

FRANCESCO CERINI

Second Year / First Semester 6BIO/07ita
118394 - INTRODUCTION TO EARTH SCIENCE

CHIARA SBARBATI

Second Year / First Semester 6GEO/05ita
121096 - MODULO B ( APPLICATION) THEORY AND APPLICATION IN BIODIVERSITY CONSERVATION - BEGINNERS - BA

GIANLUCA PIOVESAN

Second Year / Second Semester 3BIO/07ENG
121095 - MODULO A ( THEORY ) THEORY AND APPLICATION IN BIODIVERSITY CONSERVATION - BEGINNERS - BA

GIANLUCA PIOVESAN

Second Year / Second Semester 3BIO/07ENG